How do you keep enzyme assays reproducible between different lab members?

Created byJarrod Kanizay AvatarJarrod KanizayBiochemistry

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We run the same kinetic assays across several people in the group, and small differences in buffer prep, temperature, or pipetting seem to shift our results more than we'd like.

I'm trying to write a shared protocol that actually gets followed rather than just filed away.

What do you do in your lab to keep assay results consistent from person to person?

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