How do you validate an antibody before trusting it in a new assay?

Created byJarrod Kanizay AvatarJarrod KanizayImmunochemistry

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We keep running into antibodies that work in one lab's hands and fail in another, and the vendor validation data rarely covers our specific application.

I'm curious how others decide an antibody is fit for purpose before committing to a new assay.

What validation steps do you consider non-negotiable, and how much do you rely on published reports versus your own testing?

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