Development and evaluation of a lung-on-a chip device for screening novel antimicrobials

About the Project

On-going studies by our group have revealed a complex and diverse microbiota in the airways of Cystic fibrosis (CF) patients, which affects disease progression. However, mechanistic studies to determine the complex interactions between bacterial species and between bacteria and the host have been limited to date, primarily due to a lack of preclinical models that mimic in vivo conditions.

In this project, we will develop a microfluidic lung-on-a-chip model using 3D printing technologies, which will reflect in vivo conditions. This model will be used to study the interaction between a range of aerobic and anaerobic members of the lung microbiota and to determine the effects of different bacterial communities on the intact airway epithelium using cell lines and primary cells from people with CF. The model will also be used to test activity of potential novel antimicrobial therapies. This project will provide extensive training in conventional aerobic and anaerobic culture methods, molecular microbiology, tissue culture and protein biochemistry as part of an internationally renowned research team.

The successful applicant will be integrated into QUB research groups of experienced researchers with access to world-leading facilities. A wide range of techniques will be used during the project including: spectroscopic, microscopic, and mechanical analysis of the 3D printed lung-on-a-chip devices, and extensive training in conventional aerobic and anaerobic culture methods, molecular microbiology, tissue culture and protein biochemistry as part of inter-disciplinary and internationally renowned research teams.

This study will develop a preclinical model that has the potential to be used to drive improvements in clinical care of people with CF and other respiratory diseases characterised by chronic infection such as bronchiectasis and COPD. The PhD student will be encouraged to engage in a variety of impact activities, disseminate the research project findings through public talks, and participate in QUB showcase events. Examples of impact activities include: blogs or web articles, magazine articles, public lectures, school visits, oral & poster presentations (at local, national and international conferences), and publication of scientific papers in peer reviewed journals.

Medicine (26)

Funding Notes

This project is not funded; applications are welcome from self-funding candidates.