Research Assistant / Associate (Cancer Science Institute, Dr Low Jun Siong's Lab)
Job Description
A full-time Research Assistant/Research Associate in the Low laboratory is available starting effective immediately. The Low laboratory will study immunology at the interplay between cancer and infectious diseases, with a particular focus on T and B cell biology. For more information, please check out our lab website at www.junsionglow.com
Duties & Responsibilities
a) Laboratory
- Ensure that the laboratory is adequately stocked with clean glassware, chemicals, and consumables for experiments
- Responsible for the cleanliness and maintenance of equipment and the laboratory
- To assist in making buffers and restocking of consumables as required for lab members
- To ensure laboratory plasmids, cell lines and consumables are properly stored
- Maintain an up-to-date inventory and database of existing lab materials, consumables and plasticware in the lab and order as necessary
b) Research
- To help in carrying out assigned Molecular Biology and Cellular Biology experiments such as
- Plasmid cloning and PCR
- Genetic manipulation experiments in cells using lentiviruses or CRISPR-Cas9 system
- Harvest protein, RNA, or DNA from tissue, cell lines, and bacteria
- Measure protein or RNA level changes by Western blot, immunostaining, qRT-PCR
- Monitor changes cellular phenotypes by fluorescent microscopy and flow cytometry
- Long term tissue culture
- Processing of patient blood and tissue samples
- Protein production and purification
- To organize, document and present the results of the experiments in a logical manner
- To assist in supervising, training, and guiding new graduate and undergraduate students
Requirements
- University degree (biology, biomedical science, immunology, molecular biology, biochemistry, genetics, or related area)
- Ability to speak and write well in English
- Good organization skills and sterility in lab practices
Knowledge of:
- Basic principles and practices in the fields of molecular, cancer and immunobiology
- General rules for safe exposure and handling of chemical and biological hazards
- Appropriate computer operations, such as Microsoft Word, Excel, PowerPoint, ImageJ (FIJI plugin), adobe photoshop, Illustrator and Flowjo.
Interested applicants should send the following documents in the job application to lowjs@nus.edu.sg
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