Research Laboratory Tech Assoc

Dedicated and hard-working research assistant/technician sought to help with biomedical research aimed at 1) understanding the molecular and biological basis of two types of skin cancer, and 2) identifying improved approaches to treating these cancers in patients. This individual will play an important role assisting with research using various cell culture and mouse models, will help with the day-to-day operations needed to run an active research lab, and will also participate directly in experimental studies. This individual will be an integral part of a busy and exciting lab, so outstanding communication skills, careful attention to detail, and excellent organizational skills are essential. This is an entry level position.

• Growing various types of cell lines in cell culture.
• Breeding and maintaining multiple strains of transgenic mice.
• Skin sampling, DNA isolation, and PCR-based genotyping to identify transgenic mice.
• Mouse monitoring for tumor development and other endpoints.
• Treating mice with anti-cancer agents or combinations of agents.
• Harvesting mouse organs and tumors for analysis.
• Cutting sections from paraffin-embedded or frozen tissue.
• Maintaining and replenishing various lab stock items.
• Preparation of shipments for collaborators.
• Detailed record keeping.
• Ability and willingness to occasionally work after hours, if needed.
• Immunofluorescence analysis of tissue sections.
• RNA isolation and analysis.
• Protein extraction, electrophoresis, and western blotting.
• Cryopreservation and recovery of cell lines.

Bachelor's degree. Familiarity with basic lab techniques and principles related to molecular and/or cell biology. Basic lab skills, excellent organization skills, attention to details, and a strong work ethic. Outstanding communication skills and ability to work as a key contributor to team science.