Research Specialist A

Research Specialist A

University Overview

The University of Pennsylvania, the largest private employer in Philadelphia, is a world-renowned leader in education, research, and innovation. This historic, Ivy League school consistently ranks among the top 10 universities in the annual U.S. News & World Report survey. Penn has 12 highly-regarded schools that provide opportunities for undergraduate, graduate and continuing education, all influenced by Penn's distinctive interdisciplinary approach to scholarship and learning.

Job Description Summary

Under supervision, perform multiple research procedures and experiments in a lab focusing on manipulating and measuring NAD+, NADH and related metabolites in rodent and human blood, cells, or tissues. Perform molecular biology experiments such as generation of mutant DNAs. Perform biochemical experiments including expression, isolation, and purification of expressed proteins. Maintain cell lines. Perform heterologous expression of proteins and administration of metabolites in cell lines. Order and maintain laboratory supplies and equipment. Isolate tissues from mice and rats. Isolation and culture of primary hepatocytes from genetic mouse models. Breeding and Maintaining mouse colonies and genotyping

Job Description

Responsibilities:

• Perform molecular biology experiments such as generation of mutant DNAs.
• Perform biochemical experiments including assays for NAD and related metabolites in rodent or human samples.
• Maintain cell lines.
• Perform heterologous expression of proteins and administration of metabolites in cell lines.
• Order and maintain laboratory supplies and equipment.
• Isolate tissues from mice and rats.
• Other duties as assigned.

Qualifications

Bachelor's degree in biology, chemistry or molecular biology, or related field and 0-1 year lab experience or an equivalent combination of education and experience required. Experience with cellular and molecular biology techniques is strongly preferred. Must have strong organizational skills, good written and oral communication skills, and must be reliable; ability to coordinate several projects concurrently is essential. Must be able to work with mouse and rat tissues. Position contingent upon funding.