Research Specialist B
Job Description Summary
The Research Specialist B will provide support for multiple research projects related to improving gene/cell therapy in the Fraietta Laboratory in the Department of Microbiology at the University of Pennsylvania. The candidate should have knowledge in tumor immunology and preferably the fast-developing and highly interdisciplinary field of immunotherapy. He/she must be able to perform high quality polychromatic flow cytometry-based assays, tissue culture, processing of human specimens, cytokine multiplex assays and ELISA. Skills in molecular biology such as DNA/RNA extraction and analysis, standard and quantitative PCR, conventional and next-generation sequencing are desired. The candidate is expected to participate in designing and performing experiments, data analysis and presentation of research findings. The Research Specialist B will manage daily research projects, data collection and presentation as well as organization of the laboratory. He/she must be able to identify and resolve technical problems, and use analytical and decision-making skills to implement assigned projects.
Job Responsibilities
- In vitro experiments - Prepares cell culture materials, media and reagents. Performs sample processing for polychromatic flow cytometry on a regular basis, and validates and develops antibody panels for such purpose
- In vivo experiments - Oversees IACUC protocol and ordering of animals. Plans in vivo experiments. Performs tumor engraftment, T cell administration, imaging and surgical procedures
- Data Acquisition, Analysis, Summarization, Reporting/Presentation - Provides data summaries, tables, graphs and figures for reports and presentations. Performs data entry and verification into databases in support of early phase cell and gene therapy clinical trials.
- Other duties and responsibilities as assigned.
Qualifications
- Bachelor of Science and 1 to 2 years of experience or equivalent combination of education and experience is required. Previous lab experience in areas of cancer and HIV preferred. Strong background and demonstrated experience in T cell immunology and flow cytometry. Strong experience working on research teams and direct participation in research studies. Strong communication skills (verbal and written).
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