related to staff position within a Research Infrastructure?: No
Offer Description
The spatial organisation of chromatin within the cell nucleus is crucial for the regulation of gene expression, DNA replication, and genome stability. One of the major epigenetic mechanisms influencing 3D genome architecture is DNA methylation, which is regulated, among others, by TET enzymes. Altered TET1 activity has been observed in many cancers, but its consequences remain poorly understood. Our preliminary results indicate that TET1 colocalises with CTCF, is present within chromatin loops, and is enriched at sites of DNA double-strand breaks. The aim of the project is to investigate the role of TET1 in the spatial organisation of chromatin and genomic instability in healthy and cancer cells. The project will use TET1 knockout, catalytically inactive, and overexpression models.
Key responsibilities include:
- Cell culture.
- Generation of functional mutants using conventional transfection methods and/or CRISPR–Cas9 genome editing.
- Preparation of sequencing libraries, including HiChIP, ChIP-seq, and CUT&RUN.
- Analysis and interpretation of results.
- Preparation of reports and scientific figures.
- Preparation of scientific manuscripts.
Where to apply
E-mail: j.borkowska@cent.uw.edu.pl
Requirements
Research Field: Biological sciences
Education Level: Master Degree or equivalent
Skills/Qualifications
MSc degree in biology, biotechnology or related discipline. The MSc degree should be obtained before the date of employment in the project.
- Confirmed status of a PhD student (on the date of starting work in the project at the latest).
- Knowledge of molecular biology techniques, including PCR, gel electrophoresis, immunofluorescence, and related methods.
- Experience in Western blotting and/or genome editing will be considered an additional advantage.
- Good practical laboratory skills and attention to detail.
- Ability to work independently as well as collaboratively within a research team.
- Good organisational and time-management skills.
- Motivation to learn new experimental and computational methods.
- Ability to analyse and critically interpret scientific data.
- Good scientific communication skills.
- Excellent command of English, both written and spoken.
- Interest in chromatin biology, epigenetics, 3D genomics, and genome regulation.
Required documents:
Specific Requirements
- Cover letter,
- Current curriculum vitae,
- Copy of MSc certificate (or, if the MSc certificate has not been obtained yet, a certificate/document about the date of MSc defense),
- Document confirming the status of PhD Student (to be provided before starting work in the project),
- Signed information on the personal data processing,
- Contact detail to at least one person who can provide letters of recommendation.
Before entering the competition, candidates are obliged to familiarise themselves with Internal Reporting Procedure.
Languages: ENGLISH
Level: Excellent
Internal Application form(s) needed
recruitment_CeNT_rodo.pdf
English
(208.19 KB - PDF)
Download
Additional Information
Benefits
- The opportunity to work in an interdisciplinary research team combining molecular biology, genomics, bioinformatics, and data analysis.
- Participation in an innovative project focused on epigenetics and the spatial organisation of the genome.
- The opportunity to work with state-of-the-art genome-mapping technologies.
- Comprehensive scientific supervision and regular methodological support throughout the PhD project.
- Training in advanced experimental and computational methods.
- Opportunities to participate in conferences, training courses, workshops, international research visits, and scientific internships.
- Collaboration with national and international research partners.
- A supportive environment that encourages scientific discussion, skill development, and increasing research independence.
- Opportunities to contribute to high-quality publications and build a strong academic profile.
Work Location(s)
Number of offers available: 1
Company/Institute: University of Warsaw (CeNT)
Country: Poland
City: Warsaw
Postal Code: 02-097
Street: Banacha 2c
Contact
City: Warsaw
Website: https://cent.uw.edu.pl/pl/main-page/
Street: Banacha 2c
Postal Code: 02-097
.webp){kind=logo}