staff position within a Research Infrastructure?: No
Offer Description
The project will combine approaches of bioinformatics, cell biology, imaging and functional genomics. The candidate will exploit existing tools and resources for Clytia. Live imaging approaches in this highly transparent species will allow the movements of stem cells to be followed. The doctoral candidate will generate bulk and scRNAseq data from young jellyfish gonads, as well as gonozooids (specialised polyps within which the jellyfish develop), for both males and females. They will use immunohistochemical, hybridisation chain reaction (HCR), and EdU approaches to visualize GSCs, pre-meiotic cells, and meiotic germ cells in fixed tissue, and to characterize the appearance of different cell populations within the polyp colony and jellyfish. The function of identified regulator genes can be tested using CRISPR/Cas9 Knockdown.
This PhD project will build on ongoing work in the host lab focussed on the identity and fate of stem cell populations in Clytia. This work indicates that multiple stem cell populations co-exist in the jellyfish, which can be traced back to the specialised polyps that produce jellyfish, including a somatic stem cell population and a potential GSC population. The sex determination aspect is based on an older study using wild Clytia collected from the bay of Villefranche indicating that temperature plays a role in determining sex, acting on young polyp colonies and/or early-stage jellyfish. Specifically, this PhD project will: 1) Define at molecular and cellular levels the germline differentiation trajectory from GSCs to germ cells in both male and female jellyfish and polyp colonies; 2) Pinpoint where and when GSCs (or their precursors) first acquire male or female identities 3) Use freshly-generated 'bipotential' colonies (i.e. ones capable of sex-switching) in order to identify key regulatory genes controlling sexual identity within the germ cell lineage in Clytia.
Where to apply
Website: https://emploi.cnrs.fr/Offres/Doctorant/UMR7009-FREBON-052/Default.aspx
Requirements
Research Field: Biological sciences
Education Level: PhD or equivalent
Research Field: Biological sciences
Education Level: PhD or equivalent
Languages: FRENCH
Level: Basic
Research Field: Biological sciences
Years of Research Experience: None
Research Field: Biological sciences » Biology
Years of Research Experience: None
Additional Information
Website for additional job details: https://emploi.cnrs.fr/Offres/Doctorant/UMR7009-FREBON-052/Default.aspx
Work Location(s)
Number of offers available: 1
Company/Institute: Laboratoire de Biologie du développement de Villefranche-sur-Mer
Country: France
City: VILLEFRANCHE SUR MER
Contact
City: VILLEFRANCHE SUR MER
Website: http://biodev.obs-vlfr.fr
