Investigating pancreatic changes after gaseous perfusion at single cell resolution

There are an estimated 9.5 million people globally living with type 1 diabetes, and for those who have become hypoglycaemic unaware, a pancreas or islet transplant can be lifechanging by restoring the ability to manage their condition. Pancreata are one of the lowest utilised organs for transplantation, with only 16% of those available for donation in the UK being transplanted. This highlights the importance of finding ways in increase utilisation and extend graft survival.

This project will explore the cellular landscape and molecular changes that occur during gaseous oxygen perfusion (persufflation (PSF)). PSF has been shown to improve islet survival by reducing inflammation, and extending viability and glucose stimulated insulin secretion. By using single-cell RNA sequencing we would be able to look closer at the specific cells and potential mechanisms at play within the islets but also the immune and exocrine compartments in the pancreas. It will involve using transformative technologies, including single cell RNA sequencing, bioinformatics and imaging. The findings from this study could reveal potential therapeutic targets to improve pancreas and islet utilisation and graft survival.

The samples collected and data generated within this project will provide a window into the pancreas, further benefiting the work conducted by the wider DRMG group. This includes but is not limited to providing a baseline and samples for investigating pancreatitis, type 1 and 2 diabetes, and beta cell survival.

The student will be emersed in a learning environment with industry partners, clinicians, post-doctoral researchers, technicians and other students, gaining invaluable insight from all levels of research, as well as clinical and industry exposure.

The lab encourages regularly presenting at meetings and seminar series, as well as local and international conferences. This aids to develop skills in presenting and networking, as well as building confidence. These skills along with the experimental techniques learnt in this experience would not only vault them into academia beyond this degree but are also highly transferable for industry and government jobs.