Laboratory and Research Specialist II

The Laboratory and Research Specialist will conduct growth experiments with pure cultures of strictly anaerobic, hyperthermophilic and mesophilic methanogenic archaea, employing serum tubes and serum bottles, and analyze these cultures employing UV-visible spectroscopy, light microscopy and protein assays.

The Laboratory and Research Specialist will formulate and prepare appropriate growth media and cultivate strictly anaerobic, hyperthermophilic and mesophilic methanogenic archaea in these media (5-100 mL) and follow their growth by cell count as determined by use of a phase contrast microscope fitted with a Petroff-Hauser counter and optical density. The Specialist will perform heterologous protein expression experiments with recombinant hyperthermophilic and mesophilic methanogenic archaea and E. coli, estimate the levels of heterologous proteins expressed via SDS-PAGE and western blotting and purify these proteins to apparent homogeneity under aerobic and anaerobic conditions via affinity chromatography. Experiences in using an anaerobic chamber, working with strictly anaerobic methanogenic archaea are must, estimation of protein, and use of a spectrophotometer are essential.