Life Science Technician 2 (Onsite Opportunity)

This position is represented by SEIU Local 2007 and the collective bargaining agreement between the university and SEIU Local 2007 governs the terms and conditions of employment.

Life Science Technician 2 (Onsite Opportunity)

This position has been deemed critical by the School of Humanities and Sciences Dean's Office and is exempt from the hiring freeze.

The School of Humanities and Sciences (H&S) is the foundation of a liberal arts education at Stanford. The school encompasses 24 departments and 25 interdisciplinary programs. H&S is home to fundamental and applied research, where free, open, and critical inquiry is pursued across disciplines. As the university's largest school, H&S serves as the foundation of a Stanford undergraduate education no matter which discipline students pursue as a major. Graduate students work alongside world-renowned faculty to pursue and shape foundational research that leads to breakthroughs and discoveries that shed new light on the past, influence the present, and shape the future. Together, faculty and students in H&S engage in inspirational teaching, learning, and research every day.

Department Description: The Dixon lab (http://www.dixonlaboratory.com) in the Department of Biology has a position for a motivated research assistant at the Life Science Technician 2 level with interests in cancer, cell biology, or chemical biology. Our lab focuses on identifying and characterizing new cell death pathways (e.g. ferroptosis) that can potentially be activated to treat cancer. A major goal of the lab is to understand how new small molecules we have discovered in the lab can induce ferroptosis in cancer cells and seeking to understand whether this involves known or novel molecular mechanisms. Research in the lab is driven by in vitro approaches with a particular focus on the effects of different small molecules on cancer cell death.

Position Summary: This position will provide opportunities to learn a range of cell biology and chemical biology techniques, and offers a unique opportunity to collaborate with other scientists interested in genomics, biochemistry and computational biology. This is a 1 year fixed term position with the possibility of renewal.

The main responsibilities of the successful candidate will be culturing cancer cells of different genotypes and examining the effects of lethal compound treatment. In addition, follow-up studies examining the effects of deleting specific genes using CRISPR/Cas9 technology on drug-induced cell death will be pursued. Other responsibilities will include performing routine laboratory techniques such as DNA cloning, as well as maintaining excellent records of lab work, curating samples, and assisting in data analysis. This is a two-year fixed-term appointment with the possibility to renew.

This is a 100% FTE, 1-year fixed-term bargaining unit, hourly position. This position will be based on the Stanford campus.

If you believe that this opportunity is a match for your knowledge, skills and abilities, we encourage you to apply. Thank you for considering employment opportunities with the School of Humanities and Sciences.

CORE DUTIES:

• Perform animal surgical techniques such as suturing, catheterization and infusion of arteries, small organ dissection, organ perfusion and tumor implantation and passage in small animals.
• Immunohistological procedures including frozen sectioning and multi-step immunologic staining for fluorescence or enzymatic detection. Some level of histologic assessment may be required.
• Maintenance, transfer and establishment of cell lines from normal or tumor cells, preparation and bioassay of serum, screening for contamination, isolation of cell mutants, cell staining, mass production of cells and cell cloning.
• Fixation, dehydration, staining and embedding of specimens for electron microscopy; carbon coating, negative staining, shadowing, operation of electron microscope (EM) either transmission or scanning; use of an ultramicrotome with a diamond knife for routine thin sectioning; alignment of an EM where the system is automated.
• Purification and /or structural characterization of compounds for which a protocolis well established.
• Separation of cells, organelles, or molecules using density gradients.
• Radioimmunoassay of viruses, proteins, or hormone levels in cell extract according to established protocol.
• Techniques such as electrophoresis, chromatography, autoradiography, radiolabeling of animals, cell cultures, or macromolecules.
• Isolation, purification and maintenance of mammalian viruses and bacteriophages; determination of viral concentration.
• Genetic manipulation of bacteria including mutagenesis, 'phage transduction and transformation.
• Accurate documentation of experimental protocols and basic statistical analysis of data.
• Other duties may also be assigned.

EDUCATION AND EXPERIENCE:

Two years of laboratory experience or two years of college coursework in the scientific discipline a combination of equivalent education and relevant work experience.

PHYSICAL REQUIREMENTS:

• Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand.
• Ability to stand and move on hard surfaces for up to eight hours.
• Occasionally lift and handle materials up to 10 pounds
• Rarely carry/pull and/or push up to 20 pounds
• Must have correctible vision to perform duties of the job.
• Position may require repetitive motion.

WORKING CONDITIONS:

Work occurs in a research laboratory environment. Depending on research, may work in the vicinity of animals, chemicals, gasses, or hazardous materials. Expected to follow all safety requirements and procedures including wearing appropriate Personal Protective Equipment (PPE) if required.

The expected pay range for this position is $38.48 (Step 1) to $46.33 (Step 10) per hour. Placement in the pay range is subject to the applicable collective bargaining agreement.

Stanford University provides pay ranges representing its good faith estimate of the salary or hourly wage the university reasonably expects to pay for a position upon hire.