MSc by Research: Trimming the fat - Identification of de-S-acylating enzymes from plants

About the Project

Protein S-acylation is a reversible fatty acid-based post-translational modification of proteins. We have demonstrated that S-acylation affects nearly half of all membrane proteins, is a dynamic modification (cycles on and off to control protein activity or function) and impacts every aspect of plant growth and development. We have defined the enzymes that add S-acyl groups to proteins, but knowledge of how S-acyl groups are removed during de-S-acylation is lacking. Taking an activity based proteomic profiling approach we have identified a number of candidate enzymes that may be involved in removing S-acyl groups from proteins.

This project aims to test the activity of these candidates against known S-acylated proteins using biochemical and chemical biology methods to identify genuine novel de-S-acylating activities from plants.

This project will provide training in protein biochemistry (SDS-PAGE, western blotting, S-acylation assays, enzymology), chemical biology (click chemistry-based detection of S-acylation, activity-based protein profiling), plant biology (transient protein expression, plant growth and transformation) and structural biology. Projects will be based at the James Hutton Institute and University of Dundee, providing a community of over 300 plant scientists and 700 life scientists, together with cutting edge facilities.

Prospective applicants are strongly encouraged to contact Dr. Piers Hemsley (pahemsley@dundee.ac.uk) in advance of application.

Our research community thrives on the diversity of students and staff which helps to make the University of Dundee a UK university of choice for postgraduate research. We welcome applications from all talented individuals and are committed to widening access to those who have the ability and potential to benefit from higher education.