"part-time Research Technician"

We are seeking a part-time Research Technician to support laboratory research on HIV-1 glycobiology. The role includes mammalian cell culture, glycoprotein and pseudovirus production, protein purification, and sample preparation for mass spectrometry. The postholder will ensure smooth day-to-day running of experiments, accurate data recording, and high-quality outputs, while contributing to lab maintenance and collaborative projects.

Key Responsibilities

• Mammalian Cell Culture: Maintain mammalian cell lines used for glycoprotein and pseudovirus production. Perform transfections and monitor cell growth to ensure optimal conditions.
• Glycoprotein Production: Express HIV-1 envelope glycoproteins in mammalian cells. Purify proteins using nickel affinity and size-exclusion chromatography. Prepare samples for mass spectrometry analysis.
• Pseudovirus Production: Assist in the generation of HIV-1 pseudoviruses. Conduct quality checks to ensure reproducibility and consistency across batches.
• Laboratory Support: Prepare buffers, reagents, and culture media as needed. Maintain laboratory equipment and ensure compliance with safety standards. Support adherence to protocols and maintain accurate records.
• Data Management: Document procedures, sample inventories, and experimental results. Keep detailed logs for pseudovirus and glycoprotein samples.
• Collaboration: Work closely with postdoctoral researchers, PhD students, and technical staff. Provide technical support across HIV-1 glycobiology projects.

About You

Knowledge, Experience and Qualifications

You should have a degree in biochemistry, molecular biology, virology, or a related field—or equivalent experience. Experience with mammalian cell culture techniques is essential. Familiarity with protein expression and purification methods (e.g., GNL affinity) and experience in pseudovirus production and purification are highly desirable.

Teamwork and Communication

You work effectively in a collaborative team environment and communicate clearly regarding progress and priorities. Maintaining accurate written and verbal records is essential.

Planning, Organisation and Resource Management

You organise lab tasks efficiently and contribute to stock management. You seek input from colleagues to refine experimental designs and improve workflows.

Problem Solving and Initiative

You troubleshoot technical issues using established practices and resolve routine problems such as contamination or poor yields. You contribute to improving lab practices within protocols and demonstrate flexibility when learning new methods.

What We Offer

Working at the University of Southampton provides access to excellent benefits including pension schemes, generous annual leave with university closure days, and family leave arrangements. We support flexible working and welcome applications from those returning from a career break.