Job Summary- Perform solid-phase peptide synthesis (SPPS) using fluorenylmethyloxycarbonyl (Fmoc) chemistry.
- Conduct peptide cleavage, deprotection, precipitation, and lyophilization.
- Purify peptides using preparative and analytical High-Performance Liquid Chromatography (HPLC).
- Characterize synthesized peptides by Liquid Chromatography-Mass Spectrometry (LC-MS), Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS), and analytical HPLC.
- Perform peptide conjugation reactions, including: Maleimide-thiol coupling, Copper-Catalyzed Azide-Alkyne Cycloaddition (CuAAC) and Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC) click chemistry, PEGylation, and Cross-linking reactions.
- Prepare multivalent peptide vaccine constructs.
- Optimize reaction conditions to improve purity and yield.
- Maintain detailed laboratory notebooks and electronic records.
- Prepare buffers, solvents, reagents, and stock solutions.
- Manage peptide inventories, chemical stocks, and laboratory supplies.
- Assist with technology transfer and preparation of protocols for scale-up.
- Follow laboratory safety procedures and institutional chemical hygiene guidelines.
- Contribute to preparation of manuscripts, grant applications, and patent documentation when appropriate.
- Perform a wide variety of complex experiments and reports results.
- Keep organized analytical data of performed experiments.
- Provide support for equipment maintenance as needed.
- Act as a liaison to students and laboratory personnel for training.
- Maintain laboratory supply requisition and management.
- May train junior-level staff.
- May help plan experiments or short-term goals.
QualificationsEDUCATION
Required
- Bachelor's degree (preferably in science, chemistry, or a related field).
SKILLS
Required
- Proficient communication skills
Working Conditions / DemandsThe role regularly requires the ability to effectively communicate. The role frequently requires the ability to move about the work environment and to position oneself to operate laboratory equipment effectively. The person in this role must be able to perform the essential functions with or without an accommodation.
Work LocationMedical Research and Library Building
975 West Walnut Street, Suite 537
Indianapolis, Indiana 46202
Advertised Salary$20.00 - 22.75 per hour
Job ClassificationCareer Level: Part Time Employee
FLSA: Nonexempt
Job Function: Part Time Employee
Job Family: Part Time Employee
Click here to learn more about Indiana University's Job Framework.
Equal Employment OpportunityIndiana University is an equal opportunity employer and provider of ADA services and prohibits discrimination in hiring. See Indiana University Notice of Non-Discrimination here which includes contact information.
Contact UsRequest Support
Telephone: 812-856-1234
