"PhD Candidate/Student in effect of lipid asymmetry on membrane protein function"

We are passionate structural biologists at CEITEC looking for a PhD Candidate/Student in the effect of lipid asymmetry on membrane protein function.

About this position: We're excited to announce an open PhD student position in Robert Vacha's Research Group! Eukaryotic and prokaryotic plasma membranes exhibit inherent asymmetry, with distinct lipid compositions between the two leaflets. However, most studies to date have focused on symmetric bilayers. This project addresses this gap by integrating molecular dynamics simulations with experimental techniques such as solid-state NMR spectroscopy and scattering methods.

The primary aim is to elucidate the structural and dynamical properties of lipids in asymmetric membranes, particularly their interactions with integral membrane proteins, with an emphasis on bacterial lipid compositions. Initial step will involve determining lipid chain order parameters, bilayer structure, and lipid dynamics. Subsequently, lipid-protein interactions will be assessed, focusing on lipid-induced modulation of enzymatic activity in two bacterial proteins: OmpLA, a β-barrel protein, and GlpG, an α-helical transmembrane protein. The findings will offer key insights into the role of lipid asymmetry in biological membranes and its influence on membrane protein function, with potential applications in the development of lipid-targeted therapeutics and biosensors for pharmaceutical and biotechnological use.

The ideal candidate profile: Must-have: MSc degree in the fields of biophysics, soft matter physics, physical chemistry, computational chemistry, statistical mechanics, or related fields; Good English language – spoken and written. Nice-to-have: Experience with molecular dynamics simulations (with GROMACS, CHARMM, NAMD, AMBER, LAMMPS, etc.) at the atomistic or coarse-grained level; Good communication and interpersonal skills; Motivation and a collaborative mindset.

What we provide to you: Open access to CEITEC MU core facilities with state-of-the-art equipment and experienced staff; Full-time contract; An attractive salary (ERC funded position); Flexible working hours; Support for further education and professional development; Benefits package: 6 weeks of paid holiday, medical insurance, financial contribution to meal and kindergarten/pension scheme, Multisport card, mental health care, sports lessons directly at the campus; Kindergarten Elánek in Campus; Support in these areas: Welcome Office service before and after arrival; Grant Office support; Postdoc Peer Committee; CEITEC events – workshops, conferences, lectures, training, family gathering etc.

How to apply? Application deadline: 31 Dec 2025; Start date: by negotiation; Applications, including a CV, motivation letter, list of publications, and contacts for at least two references should be sent via e-application. Any troubles with the e-application, please contact Petra Linhartova (petra.linhartova@ceitec.muni.cz).