Offer Description The candidate will join a research project aimed at advancing our understanding of the role of mitochondria in
disease, with a particular focus on mitochondrial fatty acid β-oxidation (FAO) across different stages of life (lactation, adulthood, and ageing). The candidate will learn and perform advanced in vitro and in vivo experimental techniques, including: TASK 1: Management of transgenic mouse colonies and generation and characterization of animal models of FAO dysfunction. TASK 2: Primary cell cultures and organoid cultures derived from muscle and liver. TASK 3: Analysis of mitochondrial function , including oxygen consumption measurements using a Clark-type oxygen electrode and Seahorse XF24/XF96 analyzers; two-dimensional electrophoresis and blue native polyacrylamide gel electrophoresis (BN-PAGE); enzymatic activity assays of mitochondrial complexes; radiometric analysis of metabolic fluxes; mitochondrial protein import and export assays; and CRISPR-based approaches to investigate mitochondrial proteins. TASK 4: Integration of multi-omics data from preclinical models (mice) and clinical cohorts of metabolic lipid disorders. Requirements Education Level: Master Degree or equivalent. Research fields: Biological sciences, Medical sciences, Pharmacological sciences, Computer science. Skills/Qualifications The evaluation process will assess the suitability of the candidate based on the following criteria: Academic performance, based on grades obtained in the undergraduate degree and Master's degree. Experience in laboratory animal handling and possession of the training and certification required to work with laboratory animals in Spain or another EU country. Experience in techniques for assessing mitochondrial function. Experience and autonomy in the use of CRISPR/Cas9 technology. Experience and autonomy in bioinformatics. Experience in techniques for analyzing skeletal muscle and liver architecture and function (experience and autonomy in microscopy, flow cytometry, histology, and organ-specific techniques will be particularly valued). English language proficiency. Additional Information Applications to this offer must compulsory be registered through the online application system of UAM. Nevertheless, prospective applicants are encouraged to contact the PI beforehand to discuss their suitability for the position, and to seek for assistance with the online application system. Work Location Universidad Autónoma de Madrid, C/ Einstein 3, 28049 Madrid, Spain
