"PhD student: The single-molecule biophysics of ribosome cooperation"

Job Summary: In this project, you will perform innovative biophysics experiments to elucidate the dynamic action of individual ribosomes. Key methodologies include optical tweezers, single-molecule fluorescence, and selective ribosome profiling. You will visualize real-time translation activity of ribosomes, detect folding of polypeptide chains, and examine how chaperones guide this process. The aim is to understand fundamental mechanisms of protein production.

Responsibilities: Directly follow the dynamics of ribosome translation, protein folding, and assembly using optical tweezers and single-molecule fluorescence. Develop new experimental schemes, adapt biochemical protocols, analyze complex temporal data, formulate new models, and explain findings in scientific papers. Collaborate with international teams using ribosome profiling and cryo-EM techniques.

Qualifications and Requirements: Outstanding experimental physicists or chemists with an interest in single-molecule techniques, ambition for breakthrough findings, programming skills for data handling, and excellent verbal and written English skills. You must hold an MSc-degree and meet requirements for a Dutch PhD examination. Thrive in a diverse, collaborative environment.

What the Employer Offers: A full-time appointment for four years with the Netherlands Foundation of Scientific Research Institutes (NWO-I). Starting salary of €2,781 gross per month, up to a maximum of €2,968 per month, employment benefits, PhD degree from Delft University of Technology upon completion, courses for PhD students, funding for international conferences, assistance with housing, visa, transport, and furnishing for foreign students. Work in a supportive, international team at AMOLF Amsterdam.