1. The development of prime editing reagents with high activity and specificity for the precise correction of common pathogenic mutations associated with Limb Girdle Muscular Dystrophy.
2. The development of genome editing reagents for selective killing of cancer cells harboring gene amplifications.
3. The utilization of unbiased genome-wide techniques for assessing off-target events and other genomic alterations potentially associated with genome editing.
4. The translation of the developed genome editing reagents to clinical application through in vivo safety and efficacy studies in relevant mouse models.

These studies will expose the successful candidate to cutting-edge genome editor engineering approaches and the delivery of these reagents in vivo via lipid nanoparticles. The successful candidate will also learn cutting edge deep-sequencing approaches to evaluate off-target editing within the genome. They will have the opportunity to participate in meetings/conferences associated with genome editing and gene therapy, which will broaden their exposure to cutting edge research within the field. Candidates should have a Ph.D. in biology, biochemistry, chemistry or a related biomedical science. In addition, the potential candidate must have strong oral and written communication skills and be eager to present research results at national meetings. Prior experience with genome editing systems, cell culture, deep-sequencing technologies and mouse disease models is advantageous but not required.

Applicants should send a letter (via email) describing their current research activities and their interest in this position, a CV (including publications) and contact information for three references, including your Ph.D. advisor.

Address letters to Scot Wolfe (scot.wolfe@umassmed.edu).

University of Massachusetts Chan Medical School
Department of Molecular, Cell and Cancer Biology
364 Plantation Street, LRB Room 619
Worcester, MA 01605

Further details about the Wolfe laboratory can be found at:
https://www.umassmed.edu/Wolfe-Lab/
https://profiles.umassmed.edu/display/133161

1. The development of prime editing reagents with high activity and specificity for the precise correction of common pathogenic mutations associated with Limb Girdle Muscular Dystrophy.
2. The development of genome editing reagents for selective killing of cancer cells harboring gene amplifications.
3. The utilization of unbiased genome-wide techniques for assessing off-target events and other genomic alterations potentially associated with genome editing.
4. The translation of the developed genome editing reagents to clinical application through in vivo safety and efficacy studies in relevant mouse models.

These studies will expose the successful candidate to cutting-edge genome editor engineering approaches and the delivery of these reagents in vivo via lipid nanoparticles. The successful candidate will also learn cutting edge deep-sequencing approaches to evaluate off-target editing within the genome. They will have the opportunity to participate in meetings/conferences associated with genome editing and gene therapy, which will broaden their exposure to cutting edge research within the field. Candidates should have a Ph.D. in biology, biochemistry, chemistry or a related biomedical science. In addition, the potential candidate must have strong oral and written communication skills and be eager to present research results at national meetings. Prior experience with genome editing systems, cell culture, deep-sequencing technologies and mouse disease models is advantageous but not required.

Applicants should send a letter (via email) describing their current research activities and their interest in this position, a CV (including publications) and contact information for three references, including your Ph.D. advisor.

Address letters to Scot Wolfe (scot.wolfe@umassmed.edu).

University of Massachusetts Chan Medical School
Department of Molecular, Cell and Cancer Biology
364 Plantation Street, LRB Room 619
Worcester, MA 01605

Further details about the Wolfe laboratory can be found at:
https://www.umassmed.edu/Wolfe-Lab/
https://profiles.umassmed.edu/display/133161