Postdoctoral Associate - Makino Lab

Job Description

The Makino Laboratory is seeking a highly motivated Postdoctoral Associate at the Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology in Jupiter, Florida. The area of our research focuses on coronary microvascular dysfunction in diabetes and menopause, particularly in cell-cell communication, mitochondrial function, cell apoptosis/senescence, and calcium handling in the endoplasmic reticulum of endothelial and smooth muscle cells.

For related publications, please see below:

The Postdoctoral Associate responsibilities will include:

Conducting cardiovascular physiological and molecular biological experiments using dissected vessels from mouse and vascular cells freshly isolated from the tissue.
Analyzing research data using different computer software
Generation of preliminary data for grant applications.
Writing drafts of manuscripts.
Coordinating with other investigators for lab research.
Maintaining the function of lab equipment, and managing the lab research record (including methodological approaches and animal protocols).

Interested applicants should send a C.V., a Letter of Interest, and the names and contact information for three references to ay.makino@ufl.edu.

Required Qualifications

A successful candidate must have a Ph.D. in biomedical sciences, M.D., or equivalent degree.
A strong record of productivity in cardiovascular physiology (e.g., assessment of cardiac function using echocardiogram and/or catheterization in mice.
Isometric tension measurement in mouse small vessels, or electrophysiological experiments using endothelial and smooth muscle cells isolated from mouse hearts and lungs) is required.

Preferred

Theoretical and working knowledge in advanced cardiovascular physiology.
Demonstrated ability to dissect small arteries [coronary and pulmonary artery] for isometric tension experiments.
Evaluate cardiac and vascular function using the Millar system or echocardiogram in mice, and isolate heart and lung vascular endothelial and smooth muscle cells.
Aseptic techniques for cell culture is preferred.
Demonstrated ability and experience in Western Blot, immunoprecipitation, PCR, qPCR, and FACS.
Demonstrated ability to contribute to experimental design, analysis, interpretation of the results, and troubleshooting.
Demonstrated experience assisting with writing scientific manuscripts and contributing to teamwork in physiological and molecular biological experiments.
Demonstrated practical time management skills to plan and organize work assignments in an efficient and productive manner.
Demonstrated skill in working with animals and human tissues.
Demonstrated knowledge of the following specialized programs: SnapGene, GraphPad, EndNote, and MS Word/Excel/PowerPoint.
Enthusiasm for learning and a genuine interest in scientific research.
Willingness and ability to learn new skills, research methods, and scientific principles.

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