Job Description

The Makino Laboratory is seeking a highly motivated Postdoctoral Associate at the Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology in Jupiter, Florida. The area of our research focuses on coronary microvascular dysfunction in diabetes and menopause, particularly in cell-cell communication, mitochondrial function, cell apoptosis/senescence, and calcium handling in the endoplasmic reticulum of endothelial and smooth muscle cells.

For related publications, please see below:

• https://pubmed.ncbi.nlm.nih.gov/38898303/
• https://pubmed.ncbi.nlm.nih.gov/31504245/
• https://pubmed.ncbi.nlm.nih.gov/22896585/

The Postdoctoral Associate responsibilities will include:

• Conducting cardiovascular physiological and molecular biological experiments using dissected vessels from mouse and vascular cells freshly isolated from the tissue.
• Analyzing research data using different computer software
• Generation of preliminary data for grant applications.
• Writing drafts of manuscripts.
• Coordinating with other investigators for lab research.
• Maintaining the function of lab equipment, and managing the lab research record (including methodological approaches and animal protocols).

Interested applicants should send a C.V., a Letter of Interest, and the names and contact information for three references to ay.makino@ufl.edu.

Required Qualifications

• A successful candidate must have a Ph.D. in biomedical sciences, M.D., or equivalent degree.
• A strong record of productivity in cardiovascular physiology (e.g., assessment of cardiac function using echocardiogram and/or catheterization in mice.
• Isometric tension measurement in mouse small vessels, or electrophysiological experiments using endothelial and smooth muscle cells isolated from mouse hearts and lungs) is required.

Preferred

• Theoretical and working knowledge in advanced cardiovascular physiology.
• Demonstrated ability to dissect small arteries [coronary and pulmonary artery] for isometric tension experiments.
• Evaluate cardiac and vascular function using the Millar system or echocardiogram in mice, and isolate heart and lung vascular endothelial and smooth muscle cells.
• Aseptic techniques for cell culture is preferred.
• Demonstrated ability and experience in Western Blot, immunoprecipitation, PCR, qPCR, and FACS.
• Demonstrated ability to contribute to experimental design, analysis, interpretation of the results, and troubleshooting.
• Demonstrated experience assisting with writing scientific manuscripts and contributing to teamwork in physiological and molecular biological experiments.
• Demonstrated practical time management skills to plan and organize work assignments in an efficient and productive manner.
• Demonstrated skill in working with animals and human tissues.
• Demonstrated knowledge of the following specialized programs: SnapGene, GraphPad, EndNote, and MS Word/Excel/PowerPoint.
• Enthusiasm for learning and a genuine interest in scientific research.
• Willingness and ability to learn new skills, research methods, and scientific principles.