Job Description
The Nascent Transcriptomics Core at Harvard Medical School is seeking a Research Assistant III to join our team. Analysis of nascent RNA – RNA actively being made – can reveal details of transcriptional responses to drugs and other perturbations in a way that other RNA sequencing methods can miss. For this reason, nascent RNA-seq methods in conjunction with Next Generation Sequencing (NGS) technology, are quickly becoming the first choice for revealing direct targets of gene regulation and the identification of regulatory elements such as enhancers. The Nascent Transcriptomics Core isolates nascent RNA using biochemical methods and uses it to construct high-quality NGS libraries, oversees sequencing, and provides users with actionable, analyzed data. We make nascent RNA techniques and analyses available to the general research community on a fee-for-service, customer-oriented basis.
The Research Assistant III reports to the Director of the Nascent Transcriptomics Core (NTC) and is responsible for constructing high-quality nascent RNA libraries for NGS, optimizing methods for their construction, and analyzing the data. This position offers the opportunity to learn cutting-edge NGS methods and nascent RNA library construction strategies, gain experience with nascent RNA data analysis, contribute to projects of direct biomedical relevance, and provide a valuable, collaborative service to the scientific research community at Harvard Medical School and the greater Boston area.
Primary Responsibilities
- Receive and perform quality control (QC) on cells and materials from users.
- Construct NGS libraries according to established protocols.
- Perform QC on constructed libraries using qPCR, TapeStation, gel electrophoresis, etc., and submit for high-throughput sequencing.
- Analyze users' data following established pipelines and scripts. This includes: Interpret analysis results and investigate potential problems. Prepare data packages for users. Keep detailed, accurate, up-to-date records of all samples and experiments.
Secondary Responsibilities
- Maintain laboratory stocks and replace as needed.
- Maintain laboratory records.
- In consultation with the core director, devise and execute experiments to test protocol improvements.
- Advise users on sample preparation.
- Other laboratory duties as assigned by the core director.
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