University of Colorado Anschutz
Department: Biochemistry & Molecular Genetics
Job Title: Research Services Professional (Open Rank: Intermediate - Senior)
Position #00840710 – Requisition #41114
Job Summary:
A Research Services Professional position is available in the laboratory of Francisco J. Asturias, in the Department of Biochemistry and Molecular Genetics at the University of Colorado Anschutz Medical Campus. The Asturias group works on structural (cryo-EM), biochemical and mechanistic studies of macromolecular complexes involved in various essential cellular processes. The person in this position will be focused on biochemical, molecular biology, genomic, and mechanistic characterization of the transcriptional Mediator complex, with emphasis on understanding the mechanism for Mediator's role in enabling signal-induced transcription initiation. Extension of the work to participate in cryo-EM studies is also a possibility. Applicants with background in biochemistry, molecular biology, genomics, and experience in recombinant protein expression and purification would be particularly well qualified. The work will involve extensive interaction with other group members and the applicant will receive training and acquire expertise in all experimental approaches used in the Asturias lab. The position will report directly to Dr. Asturias.
The person in this position must be highly motivated and well-organized, and capable of working both independently and as a member of a research team. They will be expected to interact and collaborate with other members of the Asturias lab and with collaborators at CU Anschutz and beyond. Good troubleshooting skills and ability to interpret, organize, and communicate experimental data are a must.
Key Responsibilities:
Department: Biochemistry & Molecular Genetics
Job Title: Research Services Professional (Open Rank: Intermediate - Senior)
Position #00840710 – Requisition #41114
Job Summary:
A Research Services Professional position is available in the laboratory of Francisco J. Asturias, in the Department of Biochemistry and Molecular Genetics at the University of Colorado Anschutz Medical Campus. The Asturias group works on structural (cryo-EM), biochemical and mechanistic studies of macromolecular complexes involved in various essential cellular processes. The person in this position will be focused on biochemical, molecular biology, genomic, and mechanistic characterization of the transcriptional Mediator complex, with emphasis on understanding the mechanism for Mediator's role in enabling signal-induced transcription initiation. Extension of the work to participate in cryo-EM studies is also a possibility. Applicants with background in biochemistry, molecular biology, genomics, and experience in recombinant protein expression and purification would be particularly well qualified. The work will involve extensive interaction with other group members and the applicant will receive training and acquire expertise in all experimental approaches used in the Asturias lab. The position will report directly to Dr. Asturias.
The person in this position must be highly motivated and well-organized, and capable of working both independently and as a member of a research team. They will be expected to interact and collaborate with other members of the Asturias lab and with collaborators at CU Anschutz and beyond. Good troubleshooting skills and ability to interpret, organize, and communicate experimental data are a must.
Key Responsibilities:
- Conduct biochemical and structural studies of Mediator. This
might include work on bacterial, insect, and mammalian cell
culture, protein expression/purification, CRISPR/Cas9 experiments,
biochemical studies, genomic studies (e.g., RNA-Seq, Chip-Seq,
CUT&RUN)