University of Colorado Anschutz Medical Campus
Department: Anesthesiology
Job Title: Research Services Senior Professional
Position #00852159 – Requisition #40857
Job Summary:
The Dabertrand Laboratory in the Department of Anesthesiology investigates the control of cerebral blood flow by ion channels and calcium signals in the pericytes, endothelial cells, and smooth muscle cells that constitute the intracerebral microcirculation. We focus on the interaction between neural activity and the microvasculature in different brain regions, in physiological and small vessels disease conditions.
We are seeking an experienced PRA who will perform lab management and conduct/assist with experiments. Experiments include, but not limited to qRT-PCR, western blotting, mammalian cell culture and cell line maintenance, DNA/RNA extraction, electrophoresis, ELISA, proliferation and migration assays, Ca2+imaging, isolated and pressurized arterioles from mouse brain, patch-clamp electrophysiology on isolated cells, in vivo two-photon microscopy. Responsibilities include solutions and samples preparation, operation and general maintenance of instruments, as well as data collection and analysis. It is an opportunity to learn cutting-edge technologies and to work with investigators on high orbit projects.
The candidate should have experience in neuroscience, cardiovascular science or related fields; experience with mouse handling is required, and having microsurgery level/fine motor skills is a plus. The successful candidate should have experience in at least one of these area: molecular biology (RNA extraction, qPCR…), electrophysiology (Patch-clap, EPSP recording), and fluorescence imaging (confocal, two-photon…). This position will work in a collaborative team environment.
Key Responsibilities:
Job Title: Research Services Senior Professional
Position #00852159 – Requisition #40857
Job Summary:
The Dabertrand Laboratory in the Department of Anesthesiology investigates the control of cerebral blood flow by ion channels and calcium signals in the pericytes, endothelial cells, and smooth muscle cells that constitute the intracerebral microcirculation. We focus on the interaction between neural activity and the microvasculature in different brain regions, in physiological and small vessels disease conditions.
We are seeking an experienced PRA who will perform lab management and conduct/assist with experiments. Experiments include, but not limited to qRT-PCR, western blotting, mammalian cell culture and cell line maintenance, DNA/RNA extraction, electrophoresis, ELISA, proliferation and migration assays, Ca2+imaging, isolated and pressurized arterioles from mouse brain, patch-clamp electrophysiology on isolated cells, in vivo two-photon microscopy. Responsibilities include solutions and samples preparation, operation and general maintenance of instruments, as well as data collection and analysis. It is an opportunity to learn cutting-edge technologies and to work with investigators on high orbit projects.
The candidate should have experience in neuroscience, cardiovascular science or related fields; experience with mouse handling is required, and having microsurgery level/fine motor skills is a plus. The successful candidate should have experience in at least one of these area: molecular biology (RNA extraction, qPCR…), electrophysiology (Patch-clap, EPSP recording), and fluorescence imaging (confocal, two-photon…). This position will work in a collaborative team environment.
Key Responsibilities:
- Collaborate with and support Principal Investigators (PI) and
other stakeholders with laboratory and research functions
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