"Research Specialist C/D"

Research Specialist C/D

Job Description Summary

The Research Specialist C/D position in The Center for Cellular Immunotherapies will support research efforts in CAR T-cell therapy development by performing complex in vitro and in vivo experiments.

Job Responsibilities

In vitro experiments

• Prepare cell culture materials, media, and reagents.
• Follow established protocols and maintain accurate experimental records.
• Isolate, transduce, transfect, electroporate, expand, formulate, and cryopreserve human T lymphocytes. Perform in vitro experiments to assess T cell phenotype and function (flow cytometry and cocultuure assays).
• Perform molecular assays, optimize experimental conditions.
• Follow standardized protocols and optimize methods to establish in vivo tumor models (IP, IV, orthotopic, subcutaneous) and monitor responses to various cell therapies.
• Perform injections, tumor measurements, imaging, bleeding, necropsy, and tissue harvesting.

Data Acquisition, Analysis, Summarization, Reporting/Presentation

• Maintain organized, real-time records of experiments for traceability and reproducibility.
• Keep accurate data records into laboratory notebooks and group folders and provide data as needed.
• Present data to supervisor and in small group meetings.

General support to laboratory

• Maintain laboratory organization and inventory.
• Collaborate with team members to achieve research goals.
• Ensure compliance with ethical standards and proper handling of animals and hazardous materials.

In addition to the duties listed, the Research Specialist D will design panels and perform multiparametric flow cytometry for phenotypic characterization of engineered T cells and co-cultures; analyzes datasets using appropriate software. Design, plan and carry out molecular assays. Independently develop protocols, ensure data collected is verified, complete and consistent and properly recorded in digital folders and lab notebook. Provides data summaries, tables, graphs and figures for reports and presentations. Train students and new members on general laboratory procedures, providing advice and mentorship on technical procedures.

Qualifications

Research Specialist C

• Bachelor of Science and 3 to 5 years of experience or equivalent combination of education and experience is required.
• Demonstrated proficiency in tissue culture techniques and basic molecular biology methods.
• Extensive in vivo experience with mouse models, including NSG/syngeneic tumor modeling, intravenous (IV).
• Ability to follow established protocols accurately and maintain detailed records.
• Strong organizational skills and ability to work collaboratively in a team environment.

Qualifications preferred:

• Experience with molecular biology techniques, including gene editing and cloning.
• Familiarity with CAR T-cell manufacturing processes and human primary lymphocyte cultures.
• Experience with imaging modalities for tumor monitoring.

Research Specialist D

• Master's degree (Biology, Immunology, or a related scientific discipline) and 3 to 5 years of experience, or an equivalent combination of education and experience.
• Demonstrated proficiency in flow cytometry, including panel design, multiparametric analysis, and data interpretation.
• Extensive in vivo experience with mouse models, including NSG/syngeneic tumor modeling, intravenous (IV), intraperitoneal (IP), and orthotopic injections, tumor measurements, blood sampling, and tissue harvesting.
• Strong analytical and decision-making skills to implement assigned projects and troubleshoot technical issues.
• Ability to work independently to manage daily research projects, data collection, and laboratory organization.
• Excellent organizational and record-keeping skills; ability to manage multiple projects simultaneously.
• Strong communication skills and ability to work collaboratively in a team environment.

Preferred:

• Experience with molecular biology techniques, including gene editing and cloning.
• Familiarity with CAR T-cell manufacturing processes and human primary lymphocyte cultures.
• Experience with imaging modalities for tumor monitoring.
• Ability to analyze and present data using statistical and graphing software.

Position is contingent upon continued funding.

Job Location

Philadelphia, Pennsylvania

Department / School

Perelman School of Medicine

Pay Range

$57,500.00 - $78,000.00 Annual Rate

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