"Research Technician C"

Research Technician C

Job Profile Title: Technician, Research Laboratory C

A research technician position is available in the Folkert lab in the Department of Surgery at the University of Pennsylvania. We are seeking a highly motivated individual to work in collaboration with the Principal Investigator, students, residents and/or graduate students to study the immune response to peritoneal surface malignancies, including appendix cancer, colon cancer, and peritoneal mesothelioma. A successful candidate would be detail-oriented, highly organized, and able to work independently after appropriate training and supervision in basic lab techniques and safety. Prior wet lab experience is advantageous, although additional training will be provided. This position will be an excellent opportunity to gain experience performing basic and translational research using patient-derived specimens, offer opportunities for publication, and the chance to work in a collaborative, multidisciplinary setting.

The successful candidate will perform a broad range of routine laboratory tests and procedures to support medical and scientific research. Performs experiments and basic cell biology and immunology techniques, including cell culture, RNA extraction, cDNA synthesis, RT-qPCR, flow cytometry and histology. Collects specimens from the operating room and processes them for downstream assays including cell culture, RNA extraction, sequencing and flow cytometry. Performs mouse experiments including tumor transplants, tumor volume measurements and drug treatments. Assists senior researchers with experiments and provides laboratory support through preparation of media and reagents, equipment upkeep, supply ordering, inventory, and overall lab organization.

Job Responsibilities

• Conduct experiments and enter findings into computer
• Analyze the results of tests or experiments to ensure conformity to specifications, and generating preliminary figures from data generated towards eventual inclusion in a manuscript
• Processing samples, including collecting samples from the operating room and processing for downstream applications. Preparation and shipping of samples when needed.
• Collect blood or tissue samples from patients.
• Maintaining a detailed log/database of collected specimens.
• Maintain and passage cell cultures
• Optimize novel in vitro culture systems, including slice culture and organoid models
• Perform murine tumor transplants, tumor volume measurements and drug treatments
• Provide scientific and technical guidance for ongoing projects
• Maintain organized laboratory records and ensure compliance with safety protocols
• Maintain inventory of lab supplies, order supplies, oversee general laboratory maintenance and safety compliance, Collection and processing of human blood and tumor specimens and maintaining a database of collected specimens, Collecting and analyzing data through biological assays and microscopy, including cell culture assays, flow cytometry and RT-qPCR, Perform routine animal studies, including tumor and tissue harvests and preparations as well as drug treatments
• Other duties and responsibilities as assigned
• He/she will collaborate closely with other lab members and the Principal Investigator on projects.

Qualifications

Bachelor of Science and 0 to 1 years of experience or equivalent combination of education and experience is required.

This position is contingent upon grant funding.

Job Location: Philadelphia, Pennsylvania

Department / School: Perelman School of Medicine

Pay Range: $19.23 - $19.23 Hourly Rate

To apply, visit https://wd1.myworkdaysite.com/en-US/recruiting/upenn/careers-at-penn/job/HUP/Research-Technician-C_JR00114511