Research Technician Chouchani lab

The Research Technician position in Dr. Edward Chouchani?s Lab will assist scientists in performing scientific research of varying complexity. Duties include working with laboratory mice and conducting basic molecular biology and cell biology techniques. Basic procedures include but are not limited to: pipetting; cell and tissue culture, handling of small laboratory animals, etc. There will be continuous learning opportunities and the position will be a good stepping stone for those interested in biomedical research.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute is a leader in life changing breakthroughs in cancer research and patient care. We are united in our mission of conquering cancer, HIV/AIDS, and related diseases. We strive to create an inclusive, diverse, and equitable environment where we provide compassionate and comprehensive care to patients of all backgrounds, and design programs to promote public health particularly among high-risk and underserved populations. We conduct groundbreaking research that advances treatment, we educate tomorrow's physician/researchers, and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

RESPONSIBILITIES:

• Assisting postdocs and students with cell based assays, which will involve maintenance of cell lines and perform routine molecular and cellular biology experiments (e.g., qPCR, western blot, sequencing, plasmid purification and bacterial transformation).
• Assisting with biochemical techniques such as protein purification and quantification, enzyme assays and mass spectrometry-based protein and metabolite measurements.
• Maintenance of Chouchani Lab animal (laboratory mouse) colony, including weaning, tail biopsies, DNA preparation and genotyping by PCR.
• Placing orders for the purchase of lab consumables.
• General lab management (meaning keep things running and functional, organized and stocked).

QUALIFICATIONS:

• Bachelor?s or Master?s degree in Biology, Biotechnology, Chemistry or a related field.
• Hands-on laboratory experience in molecular and/or cell biology.
• Previous experience working in a medical or scientific research setting is preferred.
• Must be self-motivated and an independent worker with excellent communication skills (written and verbal).
• At least two years of commitment is strongly preferred.

PREFERRED QUALIFICATIONS:

• Prior experience with protein purification, structural biology, biochemical enzyme assays, and mass spectrometry
• Prior experience with T cells or other immune cells (e.g., primary T cells from PBMCs or other systems)
• Familiarity with standard molecular biology techniques (e.g., PCR, western blot, cloning)
• Experience with genetic sequencing and basic data analysis.

SUPERVISORY RESPONSIBILITIES:

No supervisory responsibilities. Normally receives detailed instructions on all work.

PATIENT CONTACT:

None