Primary Work Address: 44 Binney Street, Boston, MA, 02115

We have an opportunity for a Research Technician to join the Chouchani lab at the Dana-Farber Cancer Institute. We tend to think of metabolites as simple building blocks and fuel for biological processes. But many metabolites appear to play crucial roles in regulating living systems. This concept of metabolite signaling remains poorly understood at the biochemical level, and we still do not understand how most metabolite signals work. Edward Chouchani’s lab is developing new technologies to better understand the rules of the language of core metabolite signals. The hope is that by understanding how nature uses metabolism to regulate biological systems, we will have new opportunities for treating human disease. More information about the lab and their work can be found by visiting https://chouchanilab.dana-farber.org/

About the Research Technician role:

The Edward Chouchani Lab has an exciting new opportunity for a research technician I position. The Chouchani Lab is located at the Dana-Farber Cancer Institute and is affiliated with Harvard Medical School. This position will assist scientists in performing scientific research of varying complexity. Duties include working with laboratory mice and conducting basic molecular biology and cell biology techniques. Basic procedures include but are not limited to pipetting, cell and tissue culture, handling of small laboratory animals, etc. There will be continuous learning opportunities, and the position will be a good steppingstone for those interested in biomedical research.

What we provide:

• Ample opportunities and support for upskilling. Coworkers that assist in learning new techniques/skills.
• Mentorship opportunities.
• Opportunities for professional growth and development, as well as opportunities to make scientific contributions.
• A competitive compensation package with comprehensive health and welfare benefits.

What you’ll do:

• Assisting postdocs and students with cell-based assays, which will involve maintenance of cell lines and perform routine molecular and cellular biology experiments (e.g., qPCR, western blot, sequencing, plasmid purification and bacterial transformation).
• Assisting with biochemical techniques such as protein purification and quantification, enzyme assays and metabolite measurements.
• Maintenance of Chouchani Lab animal (laboratory mouse) colony, including weaning, tail biopsies, DNA preparation and genotyping by PCR.
• Procurement tasks such as ordering, checking off deliveries, distributing packages and maintaining stocks of consumables.
• General lab management, organization and day-to-day operations.

What you bring:

• Bachelor’s or master’s degree in biology, Biotechnology, or a related field.
• Hands-on laboratory experience in molecular and/or cell biology.
• Previous experience working in a medical or scientific research setting is preferred.
• Must be self-motivated and an independent worker with excellent communication skills (written and verbal).
• Prior experience with stem cell culture (hESCs, hiPSCs, or related systems)
• Familiarity with standard molecular biology techniques (e.g., PCR, western blot, cloning)
• Experience with genetic sequencing and basic data analysis.