Research Technician II - Pediatrics Newborn Medicine

Scheduled Hours

40

Position Summary

The newly forming Alfano lab focuses on the mechanisms of pathogen-specific molecular determinants of sepsis and endothelial dysfunction. The goal of our research is to determine the factors that confer increased risk of life-threatening sepsis to certain populations and provide novel insights in future therapeutic developments. We utilize extensive in vivo and in vitro modeling of bacterial infection in concert with both bacterial and host genetic perturbation to examine the host-bacterial interaction. Experimental approaches involve endothelial cell culture, bacterial culturing, microscopy, flow cytometry, molecular biology, functional cell-based in vitro assays, and in vivo murine models.

The Research Technician will work closely with the PI and other members of the laboratory in studies examining mouse models of infection and endothelial dysfunction. Assists with the technical aspects of studies and experiments including documentation, preparation of materials, and assisting with analysis of results. Our work operates alongside the mentorship of the Bubeck Wardenburg lab at WashU Medicine. This position offers the opportunity to join an innovative research team and contribute to basic and translational research projects.

Job Description

Primary Duties & Responsibilities:

• Assists with research studies in both in vitro and in vivo systems, experiments, and assays including collection of data, preparation of reagents, set-up and maintenance of equipment.
• Assists with the maintenance of the laboratory’s mouse colony (e.g., breeding and weaning mice, and genotyping for maintenance of genetic integrity of the colony).
• Performs data entry, maintains data files on research, and assists with analysis.
• Complies with established safety procedures and maintains required documentation on laboratory and specimen conditions.
• Ensures lab conditions and equipment are properly cleaned and maintained in accordance with established procedures.
• Assists with general lab maintenance including ordering and shipping. 

Working Conditions:

• This position works in a laboratory environment with potential exposure to biological, chemical, and BSL2 pathogens.
• The individual must be physically able to wear protective equipment and to provide standard care to research animals.

The above statements are intended to describe the general nature and level of work performed by people assigned to this classification. They are not intended to be construed as an exhaustive list of all job duties performed by the personnel so classified. Management reserves the right to revise or amend duties at any time.

Required Qualifications

Education:

Bachelor’s degree or combination of education and/or experience may substitute for minimum education.

Certifications/Professional Licenses:

No specific certification/professional license is required for this position.

Work Experience:

No specific work experience is required for this position.

Skills:

Not Applicable

Driver's License:

A driver's license is not required for this position.

More About This Job

The ideal candidate will have up to one year of experience in a laboratory setting.

Preferred Qualifications

Education:

No additional education unless stated elsewhere in the job posting.

Certifications/Professional Licenses:

No additional certification/professional licenses unless stated elsewhere in the job posting.

Work Experience:

Laboratory Setting (1 Year)

Skills:

Blotting Techniques, Cell Cultures, Cellular Biology Techniques, Cloning, Communication, Detailed Reports, Flow Cytometry, Laboratory Instrumentation, Laboratory Research, Microscopy Techniques, Molecular Techniques, Mouse Handling, Polymerase Chain Reaction (PCR), Software Program, Working Independently

Grade

R06-H