Position Description
Research Technician
- Job Category: Fulltime Term
- Exempt/Overtime Eligible: Overtime Eligible
- Benefits Eligible: Benefit Based
Job Summary
We are seeking a highly motivated and detail-oriented Research Technician with extensive hands-on experience in insect cell culture (Sf9 and Hi5) using the baculovirus expression system and mammalian cell culture across diverse cell lines. The successful candidate will support ongoing structural and functional studies of protein complexes by producing high-quality recombinant proteins in both insect and mammalian systems.
Essential Job Duties
- Generate bacterial, insect cell, and mammalian expression constructs (basic molecular cloning techniques required).
- Maintain and passage insect cells (Sf9 and Hi5) and mammalian cell cultures under sterile conditions.
- Generate and amplify recombinant baculoviruses for protein expression in Sf9 and Hi5 cells.
- Transfect and maintain various mammalian cell lines (e.g., HEK293, HTC116, Vero, HeLa) for transient and stable expression.
- Optimize expression protocols for high-yield protein production in both systems.
- Perform small- and large-scale protein expression experiments.
- Harvest cells and assist with downstream, protein purification (modern chromatography methods).
- Perform immunofluorescence microscopy to assess protein localization.
- Conduct SDS-PAGE gel analysis and western blotting, as part of routine experiments.
- Maintain detailed records of all experiments, protocols, and cell line inventories.
- Participate in general lab maintenance (organization, restocking, and generating shared reagents).
- Support researchers with other tasks as needed.
Basic Qualifications
- Bachelor’s degree in Biology, Biochemistry, Molecular Biology, or a related field.
- 2 years of hands-on experience with Sf9 and/or Hi5 cell culture and baculovirus-based protein expression.
- Proficiency in aseptic technique and routine mammalian cell culture.
- Familiarity with restriction enzyme cloning, western blotting, SDS-PAGE gel analysis, immunofluorescence microscopy, protein purification.
- Strong organizational and communication skills.
- Ability to work independently and collaboratively in a team environment.
Preferred Qualifications
- Experience scaling up insect and/or mammalian cultures using flasks.
- Familiarity with serum-free media, virus titering, and MOI optimization.
- Understanding of basic molecular cloning and transfection techniques.
- Protein purification abilities are a plus but not necessary.
- Prior experience in academic or industrial protein production environments.
Required Documents
- Resume
Hiring Range
$20.00 - $27.00 Per Hour
The salary of the finalist(s) selected for this role will be set based on a variety of factors, including but not limited to, internal equity, experience, education, specialty and training.
As one of the largest employers in Pasadena, CA, Caltech is committed to providing comprehensive benefits to eligible employees and their eligible dependents. Our benefits package includes competitive compensation, health, dental, and vision insurance, retirement savings plans, generous paid time off (vacation, holidays, sick time, parental leave, bereavement, etc.), tuition reimbursement, and more. Non-benefit eligible employees will have access to some benefits such as onsite counseling and sick time. Learn more about our benefits and staff perks.