Johns Hopkins, founded in 1876, is America's first research university and home to nine world-class academic divisions working together as one university.
We are seeking a Research Technologist who will perform laboratory tasks and completes routine assays following common or established protocols and procedures. The laboratories investigate immune-based approaches and neoantigen vaccination for prevention RAS mutation pancreatic cancer.
Specific Duties & Responsibilities
- Perform clinical and/or research laboratory experiments by following common protocols independently or with instructions.
- Use basic science knowledge to learn techniques and methods of the lab.
- Gain problem-solving skills through progressive training, understanding of the research, and experience.
- Run lab tests and complete measurements.
- Identify and resolve common problems involved in routine assays.
- Increase skill base and knowledge of lab research and relevant assays.
- Train users in standard equipment operation and routine lab techniques and procedures.
- Set-up experiments based on defined standards.
- Collect project data.
- Prepare, test, and maintain standard laboratory equipment.
- Perform related laboratory maintenance, monitor inventory of supplies, clean equipment.
- Tissue culture: Culture and maintain primary cells and cell lines using sterile technique. Perform in vitro assays to assess cellular responses and use gene editing techniques to generate transgenic cellular models.
- Maintain animal colonies and participate in mouse experiments including tumor implantation, tumor growth measurement, and harvesting of tumor and immune tissue.
- Perform immunological assays including flow cytometry, ELISA, and ELISPOT.
- Data analysis and interpretation: Analyze experimental data using statistical methods and relevant software tools, such as GraphPad, and FlowJo.
- Routine lab maintenance: Maintain lab equipment, reagent stocks, and necessary records.
- Other duties as assigned.
Minimum Qualifications
- Bachelor's Degree in Biology, Chemistry or related field
- Additional education may substitute for required experience and additional related experience may substitute for required education beyond a high school diploma/graduation equivalent, to the extent permitted by the JHU equivalency formula.
Preferred Qualifications
- Experience in a comparable laboratory, or a significant independent research project, not related to normal course requirements.
- Experience with various laboratory techniques including flow cytometry, ELISA, western blotting, and PCR.
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