Tomáš Garrigue Masaryk - Graduated in 1876, first President of Czechoslovakia, known for his role in founding the country.
Jan Evangelista Purkyně - Graduated in 1819, renowned physiologist and anatomist, known for his contributions to the fields of histology and embryology.
František Palacký - Graduated in 1818, historian and politician, often referred to as the 'Father of the Nation' in the Czech Republic.
Alena Gajdošová - Graduated in 1996, notable tennis player who reached a career-high ranking of World No. 25.