Inflammation Off-Switch: Epoxy-Oxylipins Discovery | AcademicJobs

Exploring Epoxy-Oxylipins: The Key to Taming Chronic Inflammation

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🔬 Unlocking the Body's Hidden Brake on Inflammation

Inflammation is the body's natural defense mechanism against injury, infection, or harmful invaders. When you cut your finger or catch a cold, immune cells rush to the scene, releasing chemicals to fight off threats and start healing. This acute inflammation is usually short-lived, resolving once the danger passes. However, in chronic conditions like rheumatoid arthritis, inflammatory bowel disease (IBD), or cardiovascular disease, this process goes awry. Immune cells fail to stand down, leading to persistent tissue damage and pain.

Recent groundbreaking research from University College London (UCL) has revealed a natural "off-switch" for inflammation hidden in our fat tissue. These tiny molecules, known as epoxy-oxylipins, play a crucial role in dialing back overzealous immune responses. Derived from polyunsaturated fatty acids (PUFAs) found in fats, epoxy-oxylipins guide immune cells toward resolution rather than prolonged battle. This discovery, published in Nature Communications on January 16, 2026, marks the first comprehensive mapping of their activity in humans during inflammation.Read the full study.

Understanding this mechanism could pave the way for new treatments that restore immune balance without the broad suppression seen in steroids, offering hope for millions suffering from chronic inflammatory diseases.

What Are Epoxy-Oxylipins and Where Do They Come From?

Epoxy-oxylipins are specialized pro-resolving mediators (SPMs), a class of lipid molecules produced from omega-3 and omega-6 fatty acids abundant in cell membranes and adipose (fat) tissue. Unlike pro-inflammatory eicosanoids such as prostaglandins, which fuel the fire of inflammation, epoxy-oxylipins act as firefighters, promoting cleanup and repair.

They are generated through the cytochrome P450 (CYP450) enzyme pathway. CYP450 enzymes add an oxygen atom across double bonds in PUFAs like linoleic acid (LA) or arachidonic acid (AA), forming epoxides—ring-shaped structures that are biologically active. Key examples include 12,13-epoxyoctadecenoic acid (12,13-EpOME) from LA and 14,15-epoxyeicosatrienoic acid (14,15-EET) from AA.

However, these epoxides are unstable and quickly broken down by enzymes like soluble epoxide hydrolase (sEH). Inhibiting sEH stabilizes epoxy-oxylipins, amplifying their anti-inflammatory effects. Fat tissue, particularly white adipose tissue, serves as a rich reservoir, releasing these molecules during inflammation to rein in systemic responses.

Diagram illustrating the biosynthesis of epoxy-oxylipins from fatty acids in fat tissue

Prior animal studies hinted at their potential, but this human research confirms their role, showing elevated levels in blood and inflamed tissues during the resolution phase.

The UCL Study: Methods and Breakthrough Insights

Led by Dr. Olivia Bracken and Professor Derek Gilroy at UCL's Division of Medicine, the study used innovative human models to mimic real-world inflammation without ethical concerns of live pathogens.

  • UV-killed E. coli (UV-KEc) model: Healthy male volunteers received intradermal injections into the forearm, triggering localized inflammation—pain, heat, redness, and swelling—peaking at 24 hours and resolving by 72 hours. Blisters were induced via suction for exudate (fluid) collection.
  • Drug intervention: Participants received GSK2256294, a safe sEH inhibitor already tested in humans for other conditions. Prophylactic dosing (2 hours before injection) or therapeutic (4 hours after) boosted epoxy-oxylipin levels.
  • Analysis: Mass spectrometry measured lipids; flow cytometry tracked immune cells; skin biopsies assessed tissue changes.

Key results showed epoxy-oxylipins peaking around 14 hours in the resolution phase. sEH inhibition increased 12,13-EpOME and ratios like EpOME:DiHOME (its breakdown product) by significant margins—up to twofold in plasma and tissue. Pain resolved faster (statistically significant at 24-48 hours), though visible symptoms like swelling were unaffected.

Professor Gilroy noted, "This is the first study to map epoxy-oxylipin activity in humans during inflammation. By boosting these protective fat molecules, we could design safer treatments for diseases driven by chronic inflammation."

📊 How Epoxy-Oxylipins Tame Immune Cells: The Monocyte Story

Monocytes are white blood cells that differentiate into macrophages or dendritic cells at inflammation sites. They progress from classical monocytes (CD14++CD16−, pro-inflammatory recruiters) to intermediate (CD14++CD16+, pathogenic amplifiers) and non-classical (CD14−CD16++, patrolling resolvers).

The study pinpointed epoxy-oxylipins' magic: they block the shift to intermediate monocytes, which expand during unresolved inflammation and drive chronicity. 12,13-EpOME specifically inhibits p38 mitogen-activated protein kinase (MAPK), a signaling protein that promotes this dangerous transition.

  • In blood: Intermediate monocytes dropped ~50% with sEH inhibition.
  • In tissue: UMAP clustering confirmed fewer intermediates, linked to reduced CD4+ T cells and increased T cell death, curbing amplification.
  • In vitro: Adding 12,13-EpOME to peripheral blood mononuclear cells (PBMCs) halted differentiation; p38 inhibitor losmapimod mimicked this.

This monocyte redirection prevents tissue damage while preserving immunity, a delicate balance lost in chronic diseases.

Therapeutic Promise: Repurposing sEH Inhibitors

GSK2256294 proved safe, with no adverse effects. By elevating epoxy-oxylipins, it offers a targeted approach over broad immunosuppressants. Ongoing clinical trials explore sEH inhibitors for hypertension, pain, and kidney disease, building evidence for inflammation.UCL press release.

Dr. Bracken explained, "Our findings reveal a natural pathway that limits harmful immune cell expansion and helps calm inflammation more quickly." For rheumatoid arthritis, where intermediate monocytes attack joints, this could prevent flares alongside DMARDs (disease-modifying antirheumatic drugs).

Lifestyle ties: Diets rich in PUFAs (fish oil, nuts) may naturally boost precursors, though more research needed. Academic researchers advancing this field often seek roles in research jobs or higher ed jobs to translate discoveries.

📈 Broader Impacts on Chronic Inflammatory Diseases

Chronic inflammation underlies 50% of deaths worldwide, per WHO. Intermediate monocytes are elevated in:

ConditionRole of Intermediate Monocytes
Rheumatoid ArthritisJoint destruction
Cardiovascular DiseaseAtherosclerosis plaques
Obesity/DiabetesInsulin resistance
Lupus, IBDTissue damage

Targeting epoxy-oxylipins could address root causes, improving outcomes where steroids fail long-term due to side effects like osteoporosis.

Flowchart of monocyte subsets and epoxy-oxylipin intervention

Arthritis UK hailed it: "We hope this will lead to new pain management options."

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Photo by Francesco Ungaro on Unsplash

Future Research and Actionable Steps

Next: Larger trials in patients with chronic conditions; sex differences (study male-only); dietary interventions. Professor Gilroy envisions repurposing for flares: "sEH inhibition may represent a tractable approach to treating chronic inflammation."

Stay informed via platforms like Rate My Professor for expert insights or explore higher ed career advice. Researchers, check professor jobs in immunology. Share your thoughts in the comments—what does this mean for health?

For academic opportunities in this cutting-edge field, visit higher-ed-jobs or university jobs to join the innovation.

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Frequently Asked Questions

🧬What are epoxy-oxylipins?

Epoxy-oxylipins are fat-derived lipid mediators produced from polyunsaturated fatty acids via CYP450 enzymes. They promote inflammation resolution by guiding immune cells.

🔄How do epoxy-oxylipins act as an inflammation off-switch?

They inhibit the differentiation of pro-inflammatory intermediate monocytes via p38 MAPK blockade, reducing tissue damage and accelerating resolution.

🧪What was the UCL study methodology?

Healthy volunteers received UV-killed E. coli injections; sEH inhibitor GSK2256294 boosted epoxy-oxylipins, measured via lipidomics and flow cytometry.

🏥Which diseases could benefit from this discovery?

Chronic conditions like rheumatoid arthritis, cardiovascular disease, obesity, IBD, and lupus, where intermediate monocytes drive pathology.

Is GSK2256294 safe for humans?

Yes, the sEH inhibitor was well-tolerated in the study with no adverse effects, building on prior trials for hypertension.

🥜Can diet influence epoxy-oxylipin levels?

PUFA-rich foods (fish, nuts) provide precursors, but clinical evidence is emerging. Consult a doctor for personalized advice.

🦠What role do monocytes play in inflammation?

Classical monocytes recruit; intermediates amplify damage; non-classical resolve. Epoxy-oxylipins favor resolvers.

📅When were epoxy-oxylipins first studied in humans?

This 2026 UCL study is the first to map their activity during human inflammation resolution. Explore research jobs in this field.

🚀What are next steps for sEH inhibitors?

Clinical trials in chronic inflammation patients, potentially repurposing for arthritis flares.

💧How does fat tissue contribute?

Adipose tissue stores PUFAs, releasing epoxy-oxylipins systemically during inflammation. Higher ed jobs in lipid research.

⚖️Differences from steroids?

Targeted monocyte control vs. broad suppression, fewer side effects like bone loss.