"Mascir - Chercheur Post-doc en biologie cellulaire et purification de protéines"

Job Details

Postdoctoral Researcher in Cell Biology and Protein Purification

Location: UM6P – Benguerir

Contract Type: Fixed term (Postdoctoral Position)

Availability: Immediate

Required Level: PhD in Cell Biology, Biotechnology, Biochemistry, or a related field

Main Responsibilities:

• Establishing and optimizing cell culture protocols under sterile conditions (adherent or suspension eukaryotic cell lines).
• Designing, establishing protocols and conducting recombinant protein expression experiments using eukaryotic or prokaryotic systems (HEK293, CHO, E. coli, etc.).
• Applying and optimizing transfection methods (chemical, electroporation) or viral infection techniques (adenovirus, baculovirus).
• Purifying target proteins using chromatographic techniques (affinity, ion exchange, gel filtration).
• Analyzing and characterizing purified proteins using SDS-PAGE, Western blot, ELISA, UV spectroscopy, BCA/Bradford assays, etc.
• Actively contributing to critical data analysis, protocol optimization, and the writing of scientific reports.
• Supporting the valorization of research outcomes through scientific publications and/or patent applications.
• Supervising students and interns involved in the project.

Required Technical Skills:

• Strong expertise in sterile cell culture techniques (eukaryotic cell lines).
• Proven experience in recombinant protein expression (transient and/or stable expression systems).
• Strong expertise in molecular biology techniques (nucleic acid extraction, PCR, RT-PCR, etc.).
• Proven experience in protein purification methods (FPLC/HPLC chromatography).
• Strong expertise in protein analysis tools (SDS-PAGE, Western blot, ELISA, UV spectroscopy, BCA/Bradford assays).
• Experience in animal experimentation would be an asset.

Preferred Qualities and Soft Skills:

• Scientific autonomy and strong experimental rigor.
• Strong organizational skills.
• Critical thinking and problem-solving abilities.
• Ability to work effectively in a multidisciplinary team.
• Good communication skills in both English and French (oral and written).