Departmental Overview
The Innovative Genomics Institute is a joint effort between the Bay Area's leading scientific research institutions, UC Berkeley and UC San Francisco, with affiliates at UC Davis, Lawrence Berkeley National Laboratory, Lawrence Livermore National Laboratory, Gladstone Institutes, and other institutions. The IGI's diverse group of leading scientists have powerful interdisciplinary expertise. They conduct world-class research, driven by the real possibility of using genome engineering to treat…
human diseases, end hunger, and respond to climate change.
In addition to our scientific efforts, the IGI is committed to advancing public understanding of genome engineering, providing resources for the broader community, and guiding the ethical use of these technologies.
Position Summary
The SRA assists researchers in the CRISPR Cures Technology Core to establish a next generation sequencing protocol to capture both DNA and RNA information from human cells.
Responsibilities
- PCR from genomic DNA or cDNA to amplify target genes
- Prepare next generation sequencing libraries to sequence the amplified genes or gene products
- Extract DNA and RNA from cultured human cells
- Culture human cell lines
- Analyze next generation sequencing data to identify mutations and quantify gene expression
Required Qualifications
- Demonstrated proficiency in maintaining mammalian tissue culture cells and performing nucleic acid delivery to them using off-the-shelf technologies such as electroporation and lipid-based delivery.
- Robust practical familiarity and proficiency with kit-based isolation of DNA and RNA from mammalian tissue culture cells.
- Robust practical familiarity and proficiency with the end-to-end process of using PCR to amplify specific genomic loci, including primer design and PCR condition optimization, as well as set up of PCR reactions in multiwell format.
- Demonstrated proficiency in using both agarose gel electrophoresis and PAGE to characterize PCR reaction products.
- Bachelor's degree in biology relevant major and/or equivalent experience/training.
Preferred Qualifications
- Library preparation for next generation sequencing
