MSc by Research: Modelling a prothrombotic tumour microenvironment

About the Project

The MSc by Research programme at the University of Aberdeen is for students interested in a research-intensive master's degree. It is designed specifically to enhance your skills for a PhD or research career. You can find further information about our academic requirements and MSc by Research programme structure here.

Patients with cancer are at increased risk of developing life-threatening blood clots (thrombosis). Cancer associated thrombosis (CAT) increases the risk of mortality approximately 3-fold risk compared to cancer patients without CAT and non-fatal thrombosis impacts patient quality of life. Most research on the mechanisms of the development of CAT has focused on the procoagulant (clot formation) mechanisms. For example, cancer cells and their extracellular vesicles express tissue factor (TF) and podoplanin which activate extrinsic coagulation and platelet aggregation through C-type lectin receptor 2, respectively.

However, the persistence and stability of a thrombus is determined by both coagulation and thrombus breakdown (fibrinolysis). The fibrinolytic system involves activation of the zymogen plasminogen to plasmin via cleavage by plasminogen activators. Fibrinolysis is regulated by inhibitors of plasminogen activation, plasmin and by down regulation of plasminogen binding sites on fibrin. Disruption of any of these steps can lead to hypo/hyperfibrinolysis. In normal physiology there are several cells that contain fibrinolytic proteins including platelets and endothelial cells. Tumour cells also utilise the fibrinolytic system for processes such as angiogenesis and metastasis.

This project aims to establish a breast cancer tumour microenvironment model to study the impact on platelet and endothelial regulation of fibrinolysis. We will model a proinflammatory tumour microenvironment using breast cancer cell monolayers or 3D mammospheres stimulated with proinflammatory cytokines. Co-culture models will be utilised to determine the impact on gene expression and protein release, exposure and activity in endothelial cells and platelets. The successful candidate will gain experience in cell culture, platelet isolation, haemostatic assays qPCR, Western blotting and fluorescence microscopy.

Informal enquiries are encouraged and can be directed to Dr Claire Whyte (c.s.whyte@abdn.ac.uk).

Candidate Background:

It would be an advantage to have cell culture experience but training will be provided.

We actively encourage applications from diverse career paths and backgrounds and across all sections of the community, regardless of age, disability, ethnicity, gender, gender expression, sexual orientation and transgender status, amongst other protected characteristics.

We also invite applications from those returning from a career break, industry or other roles. We typically require a minimum of a 2:1 UK Honours degree (or equivalent), but exceptions can be made where applicants can demonstrate excellence in alternative ways, including, but not limited to, performance in masters courses, professional placements, internships or employment.

APPLICATION PROCEDURE:

• Please note: This is a self-funded opportunity only.
• Prospective students should contact the lead supervisor (via the email address listed above) to discuss the research project and complete a proposal form prior to / or shortly after applying.
• Formal applications can be completed online: https://www.abdn.ac.uk/pgap/login.php
• You should apply for Medical Sciences (MSc) to ensure your application is passed to the correct team.
• Please clearly note the name of the supervisor and the project title on the application form.
• Your application must include: a personal statement, an up-to-date copy of your academic CV, and clear copies of your educational certificates and transcripts.
• If you are still undertaking your undergraduate degree, it is helpful to the selection panel if you could provide documentation showing your grades to date (this can be a screenshot from an online portal).
• Please note: Project supervisors will not respond to requests for funding assistance.
• If you require any additional assistance in submitting your application or have any queries about the application process, please don't hesitate to contact us at pgrs-admissions@abdn.ac.uk

Funding Notes

This is a self-funding project open to students worldwide. Our typical start dates for this programme are October/ February, however we may be able to accommodate start dates in other months if this is preferred.

Fees for this programme are currently £5,006 for home/UK students, and £27,300 for international students.

Additional research costs/bench fees of £3,000 will also apply to the MSc by Research programme.