The PhD will first define how NOHA behaves as a ROS scavenger and NO donor in vitro and in primary human aortic endothelial cells. It will then use quantitative proteomics to map the downstream endothelial signalling pathways modulated by NOHA under hyperglycaemic and inflammatory conditions. Finally, it will assess whether NOHA can restore endothelial function in advanced in vitro models.

The project will employ a range of techniques including cell culture, quantitative NO and ROS assays, HPLC-EC analysis of pterins, proteomics, Western blotting, angiogenesis and adhesion assays, and ECIS.

The PhD will first define how NOHA behaves as a ROS scavenger and NO donor in vitro and in primary human aortic endothelial cells. It will then use quantitative proteomics to map the downstream endothelial signalling pathways modulated by NOHA under hyperglycaemic and inflammatory conditions. Finally, it will assess whether NOHA can restore endothelial function in advanced in vitro models.

The project will employ a range of techniques including cell culture, quantitative NO and ROS assays, HPLC-EC analysis of pterins, proteomics, Western blotting, angiogenesis and adhesion assays, and ECIS.