Open Rank Laboratory Sciences Professional

Job Summary

A position as a Research Services Entry, Intermediate, or Senior Laboratory Sciences Professional is available within the Appel research laboratory in the Department of Pediatrics, Section of Developmental Biology, at the University of Colorado Anschutz. This is a full-time (1.0FTE) position that will utilize molecular, biochemical, genetic, light microscopy, and zebrafish embryological approaches in a wet lab setting.

The Appel lab studies the formation of neurons and glial cells in the developing nervous system. As a Research Services Professional in our lab, you will work closely with graduate students and postdoctoral fellows to support their projects. The work is highly flexible and variable and includes standard molecular biology techniques (PCR, subcloning, gel electrophoresis, in situ RNA hybridization, immunohistochemistry), light microscopy (fluorescence and confocal microscopy), single-cell RNA sequencing, zebrafish husbandry, and zebrafish embryological experiments.

Key Responsibilities

• Molecular cloning and PCR.
• Cell dissociation and sorting for single-cell RNA sequencing.
• Performing experiments using genetic mutants, microinjections, fluorescent in situ RNA hybridization, immunohistochemistry, and confocal microscopy.
• Manage zebrafish stocks and train others in zebrafish husbandry.
• Maintaining records of zebrafish stocks, molecular reagents, and experimental data.
• Writing reports for scientific summaries and manuscripts.
• Preparing data and figures for grant proposals and manuscripts.