"PhD student: The single-molecule biophysics of ribsomes and phase separation"

Job Summary

In this project, you will perform innovative (bio)physics experiments to elucidate the dynamic action of individual ribosomes and their relation to mRNA structure. Key methodologies include optical tweezers, single-molecule fluorescence, and selective ribosome profiling. You will visualize real-time translation activity, conformational dynamics of mRNA structure, and their interplay in the Tans biophysics lab at AMOLF Amsterdam. The aim is to elucidate fundamental mechanisms underlying protein production.

Responsibilities

You will directly follow the dynamics of ribosome translation, protein folding, and assembly using optical tweezers combined with single-molecule fluorescence. This involves developing new experimental schemes, using cutting-edge methods, adapting biochemical protocols, analyzing complex temporal data, formulating new models, and explaining findings in scientific papers. You will collaborate with teams using ribosome profiling and cryo-EM techniques to address questions about ribosome cooperation, mRNA interactions, and protein complex formation.

Qualifications and Requirements

We are looking for outstanding experimental physicists or chemists with an interest in single-molecule techniques, ambition for breakthrough findings, programming skills for data handling, and the ability to thrive in a diverse, collaborative environment. Excellent verbal and written English skills are essential. You must meet the requirements for an MSc-degree to be eligible for a Dutch PhD examination.

What the Employer Offers

The position is a full-time (40 hours/week) appointment for four years with the Netherlands Foundation of Scientific Research Institutes (NWO-I). The starting salary is €2,781 gross per month, up to a maximum of €2,968 per month, with a range of employment benefits. After completion, a PhD degree will be granted by Delft University of Technology. Several courses are offered for PhD students, funding is available for international conferences, and assistance is provided for housing, visa applications, transport costs, and furnishing expenses. You will work in an international, supportive team at AMOLF, which focuses on the physics of complex matter and functional materials.