RESEARCH FELLOW

Responsibilities:

• Establish and analyze Tgfbi knock out and transgenic mice +/- induction of type and type diabetes mellitus. This includes analyses of kidney tissue by immunohistochemistry (IHC), immunofluorescence (IF), RNA-sequencing, scRNA-seq and spatial transcriptomics; analysis or urine and blood samples.
• Perform tissue culture experiments which includes exposure of human and non-human cultured cells to recombinant TGFBI and inhibitors of TGFBI and other related molecules, and analysis of cellular phenotype by detachment / adhesion assays, Western Blotting, IHC/IF, RNA-seq, qrt-PCR, co-IP, pull-down assay and other molecular biology assays.
• Interrogate proteins in human and mouse urine and blood samples using ELISA and other molecular biology assays. Perform statistical analysis of findings (with support from biostatistician).
• Preparation of manuscripts, presentations, meeting abstracts, grant proposals.

Qualifications:
PhD in Molecular Biololgy or related fields.
Skills and experience with basic molecular methods including but not limited to processing/preserving/analyzing mammalian organ tissue (mice), culture of epithelial cells, immunohistochemistry (IHC), immunofluorescence (IF), Western Blotting, RNA-sequencing, ELISA.
Willingness and ability to perform experimental work with rodents (mice).
Experience with mouse work is preferred.

Michigan Medicine conducts background screening and pre-employment drug testing on job candidates upon acceptance of a contingent job offer and may use a third party administrator to conduct background screenings. Background screenings are performed in compliance with the Fair Credit Report Act. Pre-employment drug testing applies to all selected candidates, including new or additional faculty and staff appointments, as well as transfers from other U-M campuses.

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