Primary Work Address: Leichtag Biomedical Research Building, La
Jolla, CA, [contact details available in the full listing]
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The Sternson Lab at the University of California, San Diego
investigates how neural circuits control appetite and motivated
behavior. A major focus of the laboratory is the development of
CaRMA imaging, an experimental framework that links
large-scale measurements of neural activity in behaving animals to
post hoc molecular identification of the same recorded neurons. We
use advanced in vivo two-photon calcium imaging together with
spatial transcriptomic and circuit-mapping approaches to determine
how molecularly defined neural populations control food seeking,
consumption, and satiety. More information can be found at
https://sternsonlab.com/.
About the role:
The Sternson Lab is seeking a Research Specialist with substantial
expertise in
in vivo two-photon imaging of the mouse brain.
This person will serve as the lab’s primary technical and
scientific resource for in vivo two-photon imaging and will take
primary responsibility for the imaging component of
CaRMA
imaging.
The position combines four closely linked responsibilities:
performing in vivo calcium-imaging experiments, developing and
optimizing methods for imaging neural activity in behaving mice,
taking substantial ownership of imaging-data analysis, and enabling
other researchers in the lab to successfully apply two-photon
imaging in their experiments.
The Research Specialist will work across the full imaging workflow,
from surgical preparation and data acquisition through image
processing, quantitative analysis, and alignment of functional
imaging data with post hoc molecular information. A major part of
the role is method development: identifying technical limitations,
troubleshooting experiments, improving imaging quality and
stability, and establishing robust approaches that can be used
across projects.
This is a highly collaborative position. The Research Specialist
will work closely with lab members to design imaging experiments,
train researchers in imaging and analysis methods, and provide
ongoing technical support as two-photon imaging is incorporated
into diverse studies of neural circuit function.
We are looking for an experienced, technically creative scientist
who enjoys solving experimental problems, developing new methods,
analyzing complex datasets, and helping others use advanced imaging
approaches effectively.
What we provide:
- A competitive compensation package, with comprehensive health
and welfare benefits.
Successful projects in which the candidate participates may
lead to authorship on scientific publications.
- One-on-one mentorship.
- A vibrant and inclusive cultural environment.
- Cutting-edge research at the interface between neuroscience and
physiology.
- Opportunity for broad interactions with other labs and the
scientific community.
What you’ll do:
- Perform in vivo two-photon calcium imaging of neural population
activity in behaving mice.
- Perform and optimize surgical preparations required for chronic
imaging, including cranial windows, headplates, and GRIN-lens
implantation as appropriate.
- Develop, optimize, and troubleshoot in vivo imaging methods for
CaRMA imaging, with an emphasis on reliable high-quality data
acquisition, imaging stability, and reproducibility.
- Take substantial ownership of two-photon imaging data analysis,
including image registration, motion correction, segmentation and
signal extraction, quality control, longitudinal tracking where
appropriate, and quantitative analysis of neural population
dynamics.
- Help align functional imaging data with post hoc molecular and
cell-type information generated through CaRMA imaging.
- Work with lab members to design and implement two-photon
imaging approaches that address their scientific questions.
- Train and support other researchers in surgical preparation,
microscope operation, experimental design, data acquisition,
analysis, and best practices for in vivo two-photon imaging.
- Take primary technical responsibility for the lab’s in vivo
two-photon imaging capabilities, including microscope performance,
troubleshooting, and implementation of improvements to optical
systems, acquisition hardware, and software in collaboration with
lab members, collaborators, and vendors.
- Integrate two-photon imaging with behavioral-control and
physiological measurement systems and contribute to experimental
design, interpretation, presentations, and scientific
publications.
What you bring:
- Substantial hands-on experience with in vivo two-photon imaging
in the mouse brain and a demonstrated ability to obtain
reproducible, high-quality imaging data.
- Experience with surgical preparations for chronic mouse brain
imaging, including cranial windows and/or GRIN-lens
approaches.
- Strong experience analyzing calcium-imaging or related neural
imaging datasets, with the ability to take substantial ownership of
analysis from raw data through quantitative interpretation.
- Programming experience in Python, MATLAB, or a related
environment.
- Strong experimental troubleshooting skills and demonstrated
interest in developing and improving in vivo imaging methods.
- Ability to work independently and take ownership of an imaging
platform while collaborating closely with researchers across
multiple projects.
- Ability and interest in teaching others and helping researchers
successfully implement two-photon imaging in their
experiments.
- Experience with behavioral neuroscience, optical
instrumentation, microscope hardware/software, or integration of
imaging with behavioral-control systems is desirable.
- Advanced degree in neuroscience, biology, biomedical
engineering, physics, or a related field, or an equivalent
combination of education and research experience. PhD preferred but
not required; relevant hands-on experience is especially
important.
Physical Requirements
Remaining in a normal seated or standing position for extended
periods of time; reaching and grasping by extending hand(s) or
arm(s); dexterity to manipulate objects with fingers, for example
using a keyboard; communication skills using the spoken word;
ability to see and hear within normal parameters; ability to move
about workspace. The position requires mobility, including the
ability to move materials weighing up to several pounds (such as a
laptop computer or tablet). Work may involve possible exposure to
malodorous vapors, low dose radiation, contamination by toxic
chemicals and acids and presence of carcinogenic substances or
other hazardous materials.
Persons with disabilities may be able to perform the essential
duties of this position with reasonable accommodation. Requests for
reasonable accommodation will be evaluated on an individual
basis.
Please Note:
This job description sets forth the job’s principal duties,
responsibilities, and requirements; it should not be construed as
an exhaustive statement, however. Unless they begin with the word
“may,” the Essential Duties and Responsibilities described above
are “essential functions” of the job, as defined by the Americans
with Disabilities Act.
LI#LL1
Compensation and Benefits
Our employees are compensated from a total rewards perspective in
many ways for their contributions to our mission, including
competitive pay, exceptional health benefits, retirement plans,
time off, and a range of recognition and wellness programs. Visit
our
Benefits at HHMI site to learn more.
Hiring Pay Range
$75,200.00 - $94,000.00
Pay Type:
Annual
The posted range reflects HHMI’s good faith estimate of the
anticipated hiring salary range for this role at the time of
posting. Actual hiring compensation is determined by a candidate’s
qualifications, experience, and internal equity.
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