Flow Cytometry Operator II

Job Description

The Flow Cytometry Core Laboratory houses a wide range of shared-use instruments, including multiple analyzers and cell sorters, an imaging flow cytometer, a laser dissection microscope, as well as various ancillary equipment. The Flow Cytometry Operator II is responsible for maintaining and operating all instruments to ensure they are functioning optimally and meeting the needs of the user community. This role also includes providing user training, advising on assay design and data analysis, enforcing adherence to standard operating procedures, and troubleshooting equipment issues or coordinating with technical support as needed.

• Perform daily/weekly instrument maintenance tasks: prepare buffers, check fluidics/optics, clean system, run quality controls and maintain databases, to ensure optimal performance and reproducibility.
• Identify and troubleshoot developing instrument issues independently or otherwise tap into external technical support in a timely manner.
• Provide instrument training and operational assistance, as needed, to the core users.
• Advise new users on experimental design and sample preparation to ensure all steps of an experiment are completed successfully.
• Assist users as needed with data analysis in Diva, FlowJo 10 and 11, SpectroFlo, Leica LAS X and the ImageStream Amnis software.
• Stay up to date on the relevant science and product development in the field through the literature and other web resources.
• Perform routine administrative and housekeeping tasks such as scheduling resources, quoting and placing orders, billing, and enforcing laboratory guidelines.
• Coordinate engineer and other vendor visits.
• Organize events such as instrument DEMOs or educational seminars.
• New responsibilities may arise as the laboratory adopts new technologies and makes improvements.

Classification Minimum Requirements

Bachelor's degree in appropriate specialization and two years of flow cytometry experience. Knowledge of flow cytometry principles and basic flow cytometry experience is required.

Required Qualifications

• Bachelor's degree in appropriate specialization (Biology, Cell Biology, Immunology, Chemistry).
• Three years or more of experience with Flow Cytometry that includes operating BD analyzers and Cell Sorters.
• Proficiency in FlowJo.
• Understanding of multicolor panel design principles.

Preferred

• Master's degree in relevant field
• Experience with Spectral Flow Cytometry
• Experience with Imaging Flow Cytometry
• Good interpersonal skills