"Principal Laboratory Technician (2 Vacancies)"

Position Summary

Rutgers, The State University of New Jersey is seeking a Principal Laboratory Technician within Pediatric Hematology and Oncology at the Rutgers Cancer Institute.

Under the supervision of the Principal Investigator, the Principal Laboratory Technician will support the senior lab staff with conducting research experiments in the area of Pediatric Hematology/Oncology. The Principal Laboratory Technician will maintain accurate records, order supplies, and conduct general maintenance of the lab. The Principal Laboratory Technician will also assist in intricate and complex cell-based, animal, and human biospecimen experiments, and prepare reagents.

Essential Duties and Responsibilities

• Performs standard experiments in the lab: Microscopy (light, fluorescent, or confocal); electrophoresis (nucleic acid and protein); PCR; Western blotting; flow cytometry (multiparameter and spectral) of mouse and human specimens; DNA/RNA extraction; preparation of samples (murine and human) for ChIP-seq, ATAC-seq (bulk, single-cell, and spatial), RNA-seq (bulk, single-cell, and spatial), and other multi-omics approaches in collaboration with the appropriate institutional core facilities.
• Responsible for animal husbandry, administration of medications to mice via oral, intraperitoneal, intravenous, or other necessary routes of administration, collection of specimens (blood, cerebrospinal fluid, organs), histology, immunofluorescence, immunohistochemistry, and analysis of biomarkers.
• Cultures mammalian cell lines (e.g. murine, human, etc.) and conducting cell-based in vitro assays.
• Orders laboratory supplies and maintains a database of supply inventory.
• Responsible for packing, unpacking, storing and classification of lab supplies and specimens (mouse and human).
• Prepares culture media, reagents, stains, and stock solutions.
• Assists the PI and senior laboratory staff with experimentation and research activities.
• Assists PI and senior lab staff in preparation of manuscripts and grant applications.

Minimum Education and Experience

• Associate's degree plus two (2) years of related research experience in a laboratory environment.

Physical Demands and Work Environment

PHYSICAL DEMANDS:

• Standing, sitting, walking, talking and hearing.
• No special vision requirements.
• Lifting up to 25 lbs.

WORK ENVIRONMENT:

• Lab environment. Moderate noise.
• Exposure to animal (mouse) blood, excrement, antineoplastic drugs, and possible exposure to infectious and communicable diseases. Universal precautions are mandatory.
• Exposure to hazardous chemicals.