Research Laboratory Tech Sr

Experimental responsibilities:

• Nanoparticle characterization
• Designing and performing PCR assays
• DNA extraction and RNA extraction
• Mammalian cell culture/transfection
• DNA/plasmid cloning
• mRNA in vitro transcription
• Flow cytometry & Fluorescence microscopy
• Data analysis and preparation of data for presentation

Non-experimental responsibilities

• Purchasing of lab supplies and equipment as authorized
• Maintenance of lab equipment
• Maintaining inventory list

• BS in molecular biology, genetics, chemistry, or related area of an equivalent combination of education and experience in a related field
• Strong organizational and multi-tasking skills
• Good documentation skills
• Excellent teamwork and communication skills
• Experience with PCR assays, DNA/RNA extraction and analysis
• Experience with in vitro cell culture
• Experience with research data analysis and experimental design
• Knowledge and understanding of lab safety protocols including biological and chemical safety
• At least one year research experience

• Assessment of gene editing (CRISPR, base editing)
• Experience with confocal fluorescence microscopy
• Experience with nanoparticle characterization and isolation/purification
• Experience with or willingness to next-generation sequencing.
• Ability and motivation to learn new experimental approaches and safety protocols, and analyze and present data

Michael Triebwasser in the Department of Pediatric Hematology/Oncology seeks a highly motivated Research Laboratory Technician to assist with laboratory research.

Dr. Triebwasser works with Prof Joerg Lahann of the Biointerfaces Institute to develop novel non-viral gene delivery methods. The goal is to understand what drives effective gene delivery to specific cell types. As a pediatric hematologist, Dr. Triebwasser is focused on gene therapy for genetic blood disorders. This work involves characterizing the physical characteristics of nanocarriers and their effectiveness in blood forming cells.

Mission Statement: The lab is committed to a safe and inclusive work environment and focused on improving the field of gene therapy for blood disorders, such as sickle cell disease.