Research Specialist B/C

Job Description Summary

The Center for Epilepsy and Neurodevelopmental Disorders (ENDD) is committed to developing therapeutic approaches for genetic neurodevelopmental disorders. The Research Specialist will contribute to a fast-paced, collaborative research program dedicated to developing therapeutic approaches for STXBP1 and SynGAP1 haploinsufficiency. To support these efforts, they will execute a range of molecular, cellular, and whole animal approaches to facilitate pre-clinical translational research in primary and iPSC neurons and mice.

Job Description

Research Specialist B duties:

1. Perform primary neuron (cortical, hippocampal) and astrocyte isolation and ex vivo culture from mouse brains.
2. Differentiate and maintain iPSC neuronal cell cultures.
3. Perform compound dosing of primary neurons and mice.
4. Maintain and coordinate genetically modified mouse colonies of Stxbp1 and SynGAP1.
5. Genotype genetically modified mouse models.
6. Harvest tissues from mammalian sources.
7. Assay transcript and protein content from mammalian tissues.
8. Prepare solutions, reagents, and media as needed.
9. Order and maintain laboratory supplies and equipment related to the studies.

Research Specialist C duties:

All duties listed above, plus:

1. Work independently and with team members to manage research projects, data collection and analysis.
2. Identify and resolve technical issues.
3. Oversee aspects of lab organization.

Qualifications:

Research Specialist B:

Bachelor's Degree in (molecular) biology, neuroscience, (bio)chemistry or related field and 1-2+ year previous laboratory experience required. Willingness to routinely work with mice. Experience with animal models, molecular biology methods, cell culture and protein biochemistry required. Work with iPSC cultures preferred. Must have strong computer skills, organizational skills and ability to communicate effectively with other lab members.

Research Specialist C:

Bachelor's degree in (molecular) biology, neuroscience, (bio)chemistry or related field and 3-5 years laboratory experience required. Master's degree preferred. Experience with using and maintaining mouse models, molecular biology techniques (RNA extraction, RT-qPCR, immunoblotting) and cell culture required. Primary neuron isolation or iPSC maintenance preferred. Must have strong organizational skills, good written and oral communication skills, and must be a self-motivated and reliable team player.

This position is contingent on continued funding.

Job Location - City, State

Philadelphia, Pennsylvania

Department / School

Perelman School of Medicine

Pay Range

$50,000.00 - $78,480.95 Annual Rate