"Research Technician I-II"

Overview

Fred Hutchinson Cancer Center is an independent, nonprofit organization providing adult cancer treatment and groundbreaking research focused on cancer and infectious diseases. Based in Seattle, Fred Hutch is the only National Cancer Institute-designated cancer center in Washington. The Subramaniam Lab is seeking a Research Tech I or II to support cutting-edge research investigating mechanisms of human mRNA translation. The position will focus on supporting projects using ReLiC, a high throughput CRISPR screening approach to identify regulators of translation and other RNA metabolic processes in human cells.

Responsibilities

• Perform mammalian cell culture and genome engineering experiments to support ReLiC screening projects
• Prepare DNA libraries for high-throughput sequencing
• Conduct biochemical experiments to isolate and analyze human ribosomes
• Assist with high-throughput sequencing data analysis using computational tools
• Maintain laboratory databases and ensure accurate data recording and verification
• Identify methodological problems in research protocols and implement modifications to optimize experimental results
• Prepare written progress reports and assist with figure preparation for publications
• Conduct literature reviews to assist in determining optimal experimental methods
• Assist in the design of experiments and be responsible for the performance of individual research projects

Qualifications

Research Technician I: Bachelor's degree in related scientific field or equivalent combination of education and experience. Demonstrated scientific knowledge and basic laboratory skills.

Research Technician II: Bachelor's degree in related scientific field or equivalent, plus 2 years of experience working in a lab outside of school coursework.

Preferred Qualifications: Experience with mammalian cell culture, plasmid cloning, microscopy, basic programming, and independent research in molecular biology. Self-motivation and ability to work independently.