"Research Specialist"

Job Req ID:  115084

Research Specialist

The Johnson laboratory is hiring a highly motivated and proficient Research Specialist with a sophisticated understanding of techniques and a solid foundation in the underlying science, gained from experience with a background in neuroscience and/or molecular biology, to conduct scientific research independently, manage their own scientific projects, and/or support the projects of other lab members. The range of duties includes, but is not limited to: experimental design, project decisions, developing methodology, conducting, modifying, and troubleshooting procedures as needed, data collection and analysis. Organizational skills and attention to detail are essential. The candidate will also contribute to data reporting in the form of co-authorship on conference presentations and published research manuscripts. The Research Specialist will be part of team transplanting stem cell derived neuronal retinal ganglion cells into mouse, rats, and non-human primates with the ultimate goal of transplanting neurons in humans for vision restoration. Please visit our website- https://johnsonlabjhu.com/

Specific Duties & Responsibilities

• Day to day management of multiple mouse lines, including tailing and PCR for genotyping and maintaining database of mouse lines.
• Prepare and carry out perfusion fixation of rats or mice, with micro-dissection of ocular and/or brain tissues. Possibly assist with rabbit and non-human primate tissue dissection
• Assist in maintaining human cell cultures stem cells; purifying, characterizing and cryopreserving cells, managing/organizing cell inventories.
• Histological methods performed in this position include cryopreservation and sectioning, subsequent immunohistochemical and/or morphologic studies of animal and human donor tissues.
• Be well versed in basic molecular biology techniques- in-situ hybridization, western blotting, performing PCR/RT-PCR/qPCR.
• Confocal and multiphoton microscopy of immunohistochemical studies of explanted mouse, rat, nonhuman primates or human ocular tissues.
• Execute proper disposal of biohazardous materials, maintain laboratory conditions and other duties as assigned by lab manager
• Image capture, reconstruction, and data analysis for morphologic study using Imaris, ImageJ, Neurolucinda, and Metamorph software.
• Identify problems, propose solutions, and make decisions on research direction considering resources and lab priorities. Continuously learn new techniques and methods as required for the advancement of research goals.

Minimum Qualifications

• Bachelor's Degree in Biology, Chemistry or related field.
• One year laboratory experience performing relevant techniques.
• Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.

Classified Title: Research Specialist   

Role/Level/Range: ACRP/03/MA  

Starting Salary Range: $17.11 - $29.90 HRLY (Commensurate with experience) 

Employee group: Full Time 

Schedule: Monday-Friday, 8:30 am - 5:00 pm 

Exempt Status: Non-Exempt 

Location: School of Medicine Campus 

Department name: ​​​​​​​SOM Oph Glaucoma  

Personnel area: School of Medicine