"Pediatrics Hematology/Oncology - Project Scientist series - Nowicki Lab"

Pediatrics Hematology/Oncology - Project Scientist series - Nowicki Lab

University of California Los Angeles

Requisition Number: JPF10807

The Division of Hematology-Oncology in the Department of Pediatrics of the David Geffen School of Medicine at UCLA is seeking a Project Scientist. The appointment will be in the Project Scientist series; academic rank will be commensurate with experience.

The position requires a Ph.D. or equivalent and prior experience. This is a non-senate position.

Duties and Responsibilities include:

• Manufacturing products for clinical research according to Good Manufacturing Practice (GMP) guidelines
• Operate in a GMP regulated environment according current standard operating protocols
• Meticulous record keeping and maintenance of GMP, GTP and basic research related records and documents.
• Attend and arrange seminars, training sessions and workshops to maintain competency in evolving current GMP/GTP regulations
• Performing post-manufacturing lot-release tests: Elisa, Colony-forming Unit (CFU) assay, ELISA, Endotoxin assay, Mycoplasma assay, vector copy number (VCN) test by ddPCR
• Culture of hematopoietic progenitor cells for in vitro differentiation into T-cells for proof of principle testing of gene delivery
• Conduct experiments for FDA IND application(s)
• Conduct DNA, RNA and protein isolation and purification according current GLP research grade protocols
• Quantification of nucleic acids and proteins
• Perform PCR and qPCR
• Perform multiparametric flow cytometry to assist on-going specific NYESO-TCR research project for intracellular, surface and peptide target markers.
• Culture adhered and non-adherent mammalian cells, preparing media, aliquots and other compounds utilized in tissue culture with proper sterile aseptic technique.
• Ability to set up co-cultures for killing-assays or peptide release production-analysis
• Ability to set up experiments utilizing real time in vitro cell imaging system Incucyte
• Perform ELISA experiments in either cellular supernatants or cellular lysates normalizing results against total protein content
• Plasmid preparation, bacteria growth on competent E. coli or Stable 3 systems, positive transformants selection and sequences analysis
• Ability of align sequences on APE-software (or-equivalent) and design plasmids for plasmid-vector construction and PCR analysis
• Perform plasmid sequencing and re-sequencing
• Production of high-titer viral vectors, purification and concentration of viral supernatants for retrovirus
• Production of high-titer viral vectors, purification and concentration of viral supernatants for lentivirus
• Determination of viral titer by ddPCR on fresh and frozen supernatants
• Document results of experiments and elaboration of reports for NYESO-TCR team leader
• Perform experiments associated to survival tittering and transduction efficiency on target cells
• Design and re-design of viral vectors for large scale manufacture of GMP and GMP-comparable clinical grade virus.
• Performs other job-related duties as assigned

Interested candidates can apply online at: https://recruit.apo.ucla.edu/JPF10807