Job Information
- Organisation/Company: FUNDACION INSTITUTO INVESTIGACION SANITARIA FUNDACION JIMENEZ DIAZ
Offer Description
Researcher for carrying out the prime editing experiments necessary for the publication of the article entitled “Highly Efficient Prime Editing Correction of a Prevalent Frameshift COL7A1 Mutation in Recessive Dystrophic Epidermolysis Bullosa
Where to apply
Requirements
Research Field: Biological sciences » Other
Education Level: Master Degree or equivalent
Skills/Qualifications
Graduate in Biology and Master's degree in Biotechnology
Specific Requirements
- Experience in developing and optimizing Base and Prime editing as gene editing strategies.
- Experience in the culture and maintenance of primary cells and cell lines
- Experience in the purification and isolation of skin cells from human and murine primary samples (skin biopsies).
- Experience in molecular biology techniques, including plasmid amplification, cloning, genomic DNA, plasmid, protein, and RNA extraction, PCR, qPCR, ddPCR, Western blot, and in vitro messenger RNA synthesis.
- Experience in the characterization of generated mutations using next-generation sequencing and Sanger sequencing.
- Experience in the development of bioengineered skin equivalents.
- Experience in histological tissue processing, sectioning, and immunohistochemistry and immunofluorescence techniques.
- Experience in writing and orally presenting experimental results in English.
Languages: English - Level: Good
Languages: Spanish - Level: Good
Work Location(s)
Number of offers available: 1
Company/Institute: FUNDACION INSTITUTO INVESTIGACION SANITARIA FUNDACION JIMENEZ DIAZ
Country: Spain
Contact
State/Province: MADRID
City: MADRID
Website: https://www.fjd.es/iis_fjd
Street: Avda. Reyes Católicos s/n
Postal Code: 28040
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