Technician B

Position Summary

The technician will work with a team of researchers for the Taub Institute for Research on Alzheimer's Disease and the Aging Brain. The nature of the research is understanding cellular and molecular mechanisms of Alzheimer's disease, cognitive aging, and related disorders. He/she will also contribute conceptual as well as interpretive assistance to the development and implementation of novel ideas that involve possible therapeutic approaches to drug discovery for Alzheimer's disease. Perform other related duties as assigned

Responsibilities

• Conducting various wet lab experiments including but not limited to iPSC derived neuronal and glial cultures. Primary mouse neuronal cultures.
• Tissue processing, Western blotting, PCR and DNA gel electrophoresis, ELISA, RNA FISH, and Fluorescent immunohistochemistry /  immunocytochemistry.
• Assist the lab members in developing new therapeutic interventions including but not limited to small molecules, peptides and gene therapy.
• Maintaining animal colonies (primarily mice), genotyping and setting up breeding to generate mice of high quality, including care and feeding, cleaning of the equipment and physical surroundings, and monitoring the health of each animal.
• Learn new protocols and experimental techniques to conduct and implement novel experiments in the lab.
• Candidate is expected to pay close attention to even minor details while conducting experiments.
• Perform other related duties and responsibilities as assigned/requested.

Minimum Qualifications

Requires a bachelor's degree in chemistry, biology, physics or related science or equivalent combination of education, training, and experience.

Preferred Qualifications

• Master?s degree in biomedical sciences and at least 6 years for lab experience.
• Experienced in working with iPSC derived neuronal and glial cultures is highly desirable.
• Candidate should have an understanding of the anatomy of mouse brain as well as experience working and maintaining a fully functional wet lab, including proficiency in the biochemistry techniques of Western Blotting, PCR and gel electrophoresis, ELISA, and
  immunohistochemistry, including relevant analytical techniques.
• Experience in Alzheimer?s disease research.
• Experience with mouse husbandry in addition to perfusion, dissecting and slicing sections of the mouse brain using a cryostat.
• Proficient in cell culture techniques for primary cell lines and immortalized cells.
• Experience working with BSL2 biosafety cabinet.